About This Project
Beach nourishment (BN) is widely used to mitigate beach erosion. There is a lack of knowledge of how BN projects affect benthic microalgae (BMA), which are important primary producers. BMA may be useful bioindicators. This project aims to examine how the BN project on Tybee Island in 2014 affected community structure of BMA. Knowledge of how BNs affect BMA can inform future decisions on how and when to do BNs and what types of sediment to use.
Ask the Scientists
Join The DiscussionWhat is the context of this research?
Beaches provide unique habitats to many organisms and are critical to the enormous travel and tourism industries. Coastal erosion is becoming an increasing threat to our beaches. Climate change increases the threat of coastal erosion through sea-level rise and severe storms. Beach nourishment (BN) is the favored management technique in the US. BN does not stop erosion so it must be periodically redone. Therefore the number of BN projects is likely to increase in the future. There is a lack of research examining the effects of these BN projects on important primary producers such as benthic microalgae.
What is the significance of this project?
Extra polymeric secretions (EPS) released by benthic microalgae (BMA) may be important in inhibiting erosion by binding sediment together. BMA contribute to the base of the food chain and may also be important bioindicators as many are geographically widespread. BMA are easy and cost-effective to identify and characterize, making them ideal for environmental health assessment. BN projects differ in season and borrow sediment source. Therefore, multiple studies like this one are needed in order to identify general patterns given the seasonal and sediment differences in BN projects. Knowledge of what type of borrow sediment and which season has the least negative effects on BMA is important for future BN projects so that environmental impacts to BMA are minimized.
What are the goals of the project?
This project aims to examine how the beach nourishment (BN) project on Tybee Island in 2014 affected community structure (biomass and species composition) of benthic microalgae and follow the time course of their recovery. The main objectives of this project are: 1) To assess how the BN affected the biomass of benthic microalgae, 2) To assess the effects of the BN on the community composition of benthic microalgae, 3) To determine if and when microalgal communities recover after BN. Other objectives include assessing the effects of BN on exopolymeric secretions (EPS) and examining how changes in sediment grain size affect the benthic microalgae community.
Budget
Benthic microalgae biomass is analyzed by measuring chloropyll a (chl a) using fluorometry which requires acetone and 10 mL pipettes. To ascertain diatom community composition, genomic DNA is extracted using an Omega E.Z.N.A. Soil DNA Kit, then amplified using polymerase chain reaction (PCR). Diatom-specific primer Stram-9F and universal eukaryotic primer Euk-517R with a 5′ GC clamp, which amplify a portion of small subunit ribosomal DNA, will be used for subsequent denaturing gradient gel electrophoresis (DGGE). Primers D512for_18S and D978rev_18S, will be used to generate an amplicon library for high-throughput DNA sequencing on an Ion Torrent Personal Genome Machine at the Medical University of South Carolina’s Proteogenomics Facility. A spectrophotometer was used to measure EPS by quantifying the polysaccharides that comprise EPS using the phenol-sulfuric acid protocol which requires sulfuric acid, ethanol, and phenol.
Endorsed by
Meet the Team
Kara Pettigrew
I am a graduate student at The University of Charleston in the Masters programs for Environmental Studies and Public Administration. I have a B.S. from Wofford College where I was a biology major and minored in business.
Craig Joseph Plante
I've been a professor in the biology department at the College/University of Charleston since 1994. I've been conducting research in the field of marine microbial ecology since my graduate school days at the University of Washington and post-doctoral work at the University of Maine's Darling Marine Center.
Lab Notes
Nothing posted yet.
Project Backers
- 3Backers
- 1%Funded
- $19Total Donations
- $6.33Average Donation