- 1. Ravn, L., Christensen, A. B., Molin, S., Givskov, M., & Gram, L. (2001). Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics. Journal of Microbiological Methods, 44(3), 239–251. doi:10.1016/s0167-7012(01)00217-2
About This Project
We are developing a paper-based cell free system that detects spoiled milk. Relying on the detection of AHL molecules used by bacteria in quorum sensing, our system will be a synthetic gene network freeze-dried on paper. We hypothesize that with a working system when AHL is present the system will produce a red pigment providing a visual cue the milk is spoiled. Because spoiled milk contains bacteria producing AHL, when our system comes into contact with spoiled milk the paper should turn red.
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