Chemically induced dimerization (CID) is the process of protein dimerization in the presence of a small molecule. CID allows for highly specific molecule sensing and flexible custom protein design, offering a versatile platform for sensing molecules. However, CID is underutilized in biosensing. We hypothesize that a CID binder-based detection assay can be developed and used to accurately quantify a variety of important small molecules with applications in research, medicine, and more.