Giant pumpkins are horticultural marvels and are the product of decades of community-wide breeding and innovation. Very little is known about the genetic history of these giant squash strains and the DNA-level changes that have enabled their gargantuan size. The main objective of this study is to genetically characterize giant pumpkins and their wild ancestors using DNA sequencing. How has human selection shaped the genome of squash to produce the biggest fruit on earth?