{"model":{"id":4473,"update_text":"

Culturing Neural Stem Cells

Stem cell culture is similar to growing and maintaining other cells - the key is to just be very careful. It's important not to be tough on the cells at any step of the culturing/passaging, even when moving the flask around. This may change the cells phenotype (what they present and behave as), which is critical to maintain.

To culture neural stem cells, I follow the neurosphere method. This requires first breaking up the cells from clumps using trypsin. After the cells are in a single cell suspension, I add chemicals to maintain their health, and re-suspend them in a new culture flask. The cells then being to clump together as they grow and divide, forming a sphere (which is why the method is called the neurosphere method). When the spheres become too large, the cells in the middle cant receive the nutrients they need, so I passage the cells fore they start dying. This method has worked well, and I have several lines of healthy and functional neural stem cells ready to go for the experiments!

","title":"Culturing Neural Stem Cells","backers_only":false,"published_at":"2016-01-28T15:00:00.803-08:00","view_count":42,"publish_on":null,"url":"https://experiment.com/u/IJ450w","image":null,"project_title":"Affordable 3D-Printed Neural Devices for Spinal Cord Regeneration","project_link":"https://experiment.com/projects/affordable-3d-printed-neural-devices-for-spinal-cord-regeneration","project_id":"affordable-3d-printed-neural-devices-for-spinal-cord-regeneration","user_id":36550,"likes":0,"comments":0,"save_url":"/updates/save","editor":"redactor","citations":[],"new_record":false,"is_published":true,"is_draft":false,"save_path":"/updates/save","file_attachments":[],"tags":["Method","Protocol"],"text_to_be_jsonified_for_read":"

Culturing Neural Stem Cells

","project_milestones":[],"completed_milestones":[],"edit_milestones_path":"/projects/affordable-3d-printed-neural-devices-for-spinal-cord-regeneration/wizard#milestone","base_path":"/projects/affordable-3d-printed-neural-devices-for-spinal-cord-regeneration/updates/IJ450w","class_name":"Update","json_for_read":{"document":{"data":{},"key":"b56f07c7f62042babf00b67c8e81bd00","kind":"document","nodes":[{"data":{},"key":"7d78ecab18f1464287e1db7ba6717fad","kind":"block","isVoid":false,"type":"heading-one","nodes":[{"key":"91af1ef00c49436a80aea4be1b502564","kind":"text","ranges":[{"kind":"range","text":"Culturing Neural Stem Cells","marks":[]}]}]},{"data":{},"key":"05d007689ed844f3b4f727a76049ea3d","kind":"block","isVoid":false,"type":"paragraph","nodes":[{"key":"e589f7c99e90495db2cd64bb783c2a5e","kind":"text","ranges":[{"kind":"range","text":"Stem cell culture is similar to growing and maintaining other cells - the key is to just be very careful. It's important not to be tough on the cells at any step of the culturing/passaging, even when moving the flask around. This may change the cells phenotype (what they present and behave as), which is critical to maintain.","marks":[]}]}]},{"data":{},"key":"2f7653c16f0d445fbc7022e5cc2ef129","kind":"block","isVoid":false,"type":"paragraph","nodes":[{"key":"2a8d89c6188a48abbb87a196fb0f12d1","kind":"text","ranges":[{"kind":"range","text":"To culture neural stem cells, I follow the neurosphere method. This requires first breaking up the cells from clumps using trypsin. After the cells are in a single cell suspension, I add chemicals to maintain their health, and re-suspend them in a new culture flask. The cells then being to clump together as they grow and divide, forming a sphere (which is why the method is called the neurosphere method). When the spheres become too large, the cells in the middle cant receive the nutrients they need, so I passage the cells fore they start dying. This method has worked well, and I have several lines of healthy and functional neural stem cells ready to go for the experiments!","marks":[]}]}]}]},"kind":"state"},"json_for_write":{"document":{"data":{},"key":"b56f07c7f62042babf00b67c8e81bd00","kind":"document","nodes":[{"data":{},"key":"7d78ecab18f1464287e1db7ba6717fad","kind":"block","isVoid":false,"type":"heading-one","nodes":[{"key":"91af1ef00c49436a80aea4be1b502564","kind":"text","ranges":[{"kind":"range","text":"Culturing Neural Stem Cells","marks":[]}]}]},{"data":{},"key":"05d007689ed844f3b4f727a76049ea3d","kind":"block","isVoid":false,"type":"paragraph","nodes":[{"key":"e589f7c99e90495db2cd64bb783c2a5e","kind":"text","ranges":[{"kind":"range","text":"Stem cell culture is similar to growing and maintaining other cells - the key is to just be very careful. It's important not to be tough on the cells at any step of the culturing/passaging, even when moving the flask around. This may change the cells phenotype (what they present and behave as), which is critical to maintain.","marks":[]}]}]},{"data":{},"key":"2f7653c16f0d445fbc7022e5cc2ef129","kind":"block","isVoid":false,"type":"paragraph","nodes":[{"key":"2a8d89c6188a48abbb87a196fb0f12d1","kind":"text","ranges":[{"kind":"range","text":"To culture neural stem cells, I follow the neurosphere method. This requires first breaking up the cells from clumps using trypsin. After the cells are in a single cell suspension, I add chemicals to maintain their health, and re-suspend them in a new culture flask. The cells then being to clump together as they grow and divide, forming a sphere (which is why the method is called the neurosphere method). When the spheres become too large, the cells in the middle cant receive the nutrients they need, so I passage the cells fore they start dying. This method has worked well, and I have several lines of healthy and functional neural stem cells ready to go for the experiments!","marks":[]}]}]}]},"kind":"state"},"slate_flag":true,"text_to_be_jsonified_for_write":"

Culturing Neural Stem Cells

","private":false},"initialComments":[]}