Apr 04, 2023
I have ordered a gene fragment of a reportered silicase enzyme: Methanosarcina thermophila TM-1 carbonic anhydrase gene. I have included adapted sequences on the ends for gibson assembley into a pBAD vector.
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Rock weathering is a process whereby atmospheric CO2 reacts with silicate minerals, promoting formation of C-rich carbonate minerals. Speeding up this process has the potential to capture up to 1.25 tonnes of atmospheric CO2 per tonne of input. However, natural processes are slow. We propose to use protein engineering, discovery of naturally evolved pathways, and bioinformatics to identify and optimize enzymes capable of accelerating mineral weathering for CO2 capture.
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