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Published on Oct 22, 2014
September 5th
Aim : Pick colonies and inoculate into PYE media Results : NA.
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Published on Oct 22, 2014
September 2nd
Aim : Transform expression vector into electrocompetent Caulobacter cells. Results : Colonies successfully grew on PYE plate with chloramphenicol.
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Published on Oct 22, 2014
August 27th
Aim : Make electrocompetent Caulobacter cells using the electrocompetent cell production protocol. Results : NA.
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Published on Oct 22, 2014
August 22th
Aim : Ligate metal binding DNA sequence into RsaA expression vector. Results : NA.
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Published on Oct 22, 2014
August 20th
August 20th Aim : Phosphorylate metal binding DNA sequence from Dunbar lab using Oligonucleotide Phosphorylation protocol prior to ligation. Double digest RsaA with PstI and BglII. Results : NA.
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