About This Project

Hands-on DNA synthesis is inaccessible outside advanced labs. We hypothesize that a safe, low-cost DNA printer using terminal deoxynucleotidyl transferase (TdT) can replicate the stepwise assembly of sequences with non-functional DNA. I will prototype and test whether the device can reliably produce short polymers to demonstrate synthesis principles. If successful, it will provide an open-source tool for teaching synthetic biology worldwide and may enable alternative DNA sequencing methods.

Ask the Scientists

What is the context of this research?

This project sits at the intersection of synthetic biology, education, and accessibility. Traditional DNA synthesizers are expensive and complex, limiting hands-on learning to well-funded labs. Our goal is to democratize access by developing a low-cost, open-source DNA synthesizer that replicates core chemical processes safely without producing active genetic material. This device provides students, educators, and independent researchers with a practical tool to understand and experiment with DNA synthesis, fostering innovation and inspiring the next generation of scientists outside traditional institutions.

What is the significance of this project?

This project means making DNA synthesis something everyone can experience firsthand, not just a select few with expensive lab access. It’s about opening the door to learning and discovery by creating a tool that’s affordable, safe, and easy to use. I believe hands-on experience sparks real curiosity and understanding, so this synthesizer can inspire students, hobbyists, and researchers alike to explore synthetic biology in new ways. By breaking down barriers, we’re helping grow a community that can innovate and learn together outside traditional labs.

What are the goals of the project?

The project aims to build a benchtop DNA printer using terminal deoxynucleotidyl transferase (TdT) to assemble short, non-functional DNA polymers. Scientific goals include assessing sequence assembly accuracy by measuring stepwise nucleotide addition, evaluating reproducibility across multiple runs, and comparing results with positive controls (commercially synthesized oligos) and negative controls (no enzyme or nucleotide). Device performance will be characterized using gel electrophoresis and spectrophotometry to quantify polymer length and yield. Key experimental variables, including reaction time, nucleotide concentration, and fluid delivery rate, will be systematically tested to optimize reliability. The device’s output will also be benchmarked against commercial synthesis methods to rigorously evaluate its potential as an educational tool.