Methods
Summary
Project will use cutting-edge techniques and protocols in synthetic biology, environmental simulation, and bioengineering to create resilient, Mars-ready lichen. The methods are designed to ensure reproducibility and scalability for future research.
1. Sample Acquisition and Selection: We will source extremophile lichen species from Earth’s harshest environments (e.g., Antarctic deserts, high-altitude regions) to identify candidates with natural resilience to cold, radiation, and desiccation.
2. Genetic Engineering: Using CRISPR-Cas9 and advanced bioinformatics, we will modify the lichen to enhance tolerance to Mars-like conditions. Key targets include:
- Increased UV resistance through melanin production.
- Improved CO₂ absorption and oxygen production via optimized photosynthesis pathways.
- Enhanced desiccation and temperature resistance through gene inserts from other extremophiles.
3. Environmental Simulation: Engineered lichen will be tested in controlled environments that replicate Martian conditions, including:
- Low-pressure chambers for atmospheric simulation.
- UV radiation setups to mimic solar exposure.
- Temperature-controlled growth chambers replicating Mars’ diurnal cycles.
4. Data Analysis and Optimization: Tools like spectrophotometry and gas exchange systems will quantify photosynthetic efficiency, CO₂ utilization, and oxygen output. Iterative testing will refine genetic modifications.
5. Documentation and Transparency: The entire process will be documented in detail, including protocols and results, to ensure the research can be reproduced by other teams.
Reproducibility Resources
- Protocols and data will be shared via open-access platforms.
- Research findings will include video demonstrations and detailed reports to guide future applications.
Protocols
This project has not yet shared any protocols.
