Discovering and Assessing the Genetic Diversity of The Olive Ridley Sea Turtle

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About This Project

This research focuses on collecting DNA samples from the olive ridley sea turtle, Lepidochelys olivacea. We are wanting to further comprehend the health of the species outside of population size by assessing the genetic diversity. High enough levels of inbreeding can cause a decrease in genetic diversity that is difficult for a species to recover from. Understanding if there are genetic factors challenging this turtle's survival will help motivate countries to find sustainable solutions.

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What is the context of this research?

The olive ridley sea turtle, previously an endangered species, is currently listed as vulnerable by the IUCN Red List. While the numbers of some sub-populations have rebounded, the species as a whole is still in steady decline due to poaching of sea turtle eggs a lack of nesting habitat. As the most abundant sea turtle in the world, these turtles are overlooked in favor of rarer sea turtle species in terms of research. We hope our research will help diagnose and assess the health of the species outside of their numbers. Our results will lay the foundation for more efforts to assist this species before its population declines to unsustainable levels.

What is the significance of this project?

Arribadas (mass nesting locations) are key to successful sea turtle reproduction. Several countries have recognized the significance of these nesting grounds, like India, which recently enacted laws preserving the country’s turtle nesting areas. Costa Rica has enacted a sustainable way of harvesting turtle eggs that maintains the current sea turtle population. Mexico also has prominent turtle nesting beaches, but has yet to take significant measures to preserve their populations. The exact opposite is actually occurring as poaching the eggs is considered economically and culturally valuable. If our research is able to prove the real danger these turtles are under, we may be able to convince and motivate Mexico to provide sustainable protection for this species.

What are the goals of the project?

We want to take genetic material from mothers and offspring at the end of the breeding season. Using this we will extract DNA and ascertain allele frequencies. We hope to produce a comprehensive picture of the current trends in genetic diversity found in the olive ridley sea turtles of Mexico. As this species is able to live up to 50 years we hope to get DNA from multiple generations. As their decline began in the 1960’s the turtles present in Mexico will be the best specimens as they have not received protection like other populations. Based off the genetic diversity and possible inbreeding of the turtles we will be able to understand the health of the species in the Eastern Pacific.

Budget

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Our funding consists of the travel expenses and supplies we will need to extract and analyze DNA on a spawning population of L. olivacea. Our methods were designed to use tools and supplies that are both cost effective and would work well on this species.

Disposable biopsy punches are an affordable and safe way of attaining genetic material from an organism. DNEasy kits are a cost-effective way for us to extract and assess DNA and blood from our samples. These are also useful kits as one can conduct many samples and give a high-yield.

Project Timeline

Ideally this project will be completed within a year of being funded. We intend that the initial fieldwork will take place during the summer of 2018. DNA extraction and analysis will be the most time consuming aspect of this project. Beyond this timeline, we are committed to further outreach and awareness efforts after our results are published.

Mar 04, 2018

Travel to La Escobilla beach in Mexico — 3 days

Jul 01, 2018

Preparation for trip — 1 month

Aug 07, 2018

Obtained biopsy material from turtles — 3-4 weeks

Oct 07, 2018

DNA extraction and analysis — 2 months

Dec 07, 2018

Final review of results/prepare for publication — 2 months

Meet the Team

Frankie Lewis
Frankie Lewis
undergraduate

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University of Oregon
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Ryan Lobrovich
Ryan Lobrovich
undergraduate

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University of Oregon
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Maisie Bailey
Maisie Bailey
Undergraduate

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University of Oregon
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Frankie Lewis

Frankie is a biology major and anthropology minor at the University of Oregon. His interests include genetics, human evolution and conservation ecology. He also writes for the Daily Emerald newspaper about local science topics and campus culture/news.

Ryan Lobrovich

Ryan is a biology major with a folklore minor at the University of Oregon. He is most interested in conservation biology, epidemiology, and botany. His current hobby is podcasting recent and relevant scientific news.

Maisie Bailey

Maisie is currently an undergraduate at the University of Oregon studying biology with a minor in chemistry. She has experience with research in cyanobacteria and had the opportunity to travel to Yellowstone National Park for field work. She hopes to continue with work in conservation biology, botany, and social justice.


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