Out for blood: Hemoparasites in white-tailed deer from the Shenandoah Valley in Northern Virginia

California State University
Chico, California
BiologyEcology
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About This Project

Our research question centers about the prevalence and diversity of hemoparasites that infect ungulate poplulations in the Northern Shenandoah Valley of Virginia. We hypothesize that ungulate populations in the Northern Shenandoah Valley harbor a high prevalence and diversity of hemoparasites which would reflect an endemic parasite assemblage associated with the ecology of the region.

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What is the context of this research?

White-tailed deer are highly prevalent in the northeastern part of the USA. There, they play a crucial role in healthy ecosystems. Unfortunately, they can also be harmful as an overabundant species threatening the environmental balance of the region (Ripa et al.,2023). Due to their prevalence, white-tailed deer are readily accessible for ecological studies which can be conducted as collaborative projects with local wildlife agencies. Recent regional surveys in the northeast of the USA have documented a significant presence of harmful organisms amongst the white-tailed deer population. Babesia, Plasmodium, and other blood parasites could be detected (Rochlin et al., 2025). These parasites have demonstrated to negatively affect host health and lifespan and further potential to transmit to livestock (Guggisberg et al., 2018). We expect a high prevalence and diversity of hemoparasites in ungulate blood due to roaming behavior in vegetation, where exposure to vectors is common.

What is the significance of this project?

Research on wildlife diseases usually focuses on broader regions, such as states or multi-state areas, rather than single protected areas with free-ranging species such as white-tailed deer. Multi-pathogen surveillance is important to monitor the health of the white-tailed deer populations in a specific region such as the Shenandoah Valley. This study aims to collect blood samples from individual white-tailed deer in that area in order to evaluate and categorize the presence and prevalence of various hemoparasitic infections.

What are the goals of the project?

The aim of our study is to conduct a systematic and comprehensive survey of all blood parasites that infect white-tailed deer in the Shenandoah Valley. We intend to provide a broad ecological profile of each detected hemoparasite species by tracking their prevalence in selected locations. The number of deer can vary. We aim for at least 50 deer but we take what we can get and are happy to sample more if given the opportunity. We have 2 official sampling sites in Front Royal Virginia, but hunters bring their deer from various locations in the Shenandoah Valley. The data generated in this study will help us map the regional concentration of each discovered blood parasite. Future sampling trips will help us to expand our generated map. Investigating the blood of livestock in the same area will further allow us to assess the presence of blood parasites in domesticated ungulates and compare the identified species to the species we have identified in wild ungulates.

Budget

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These funds are meant to support a project that I hope to prime for my master’s thesis in fall 2026. I intend to use the funds to fly round-trip from Sacramento (CA) to Dulles (VA) and to reach the sampling site at Front Royal (VA). The QIAamp DNA Micro Kit will be used to extract genomic DNA from the sampled dried deer blood. The extracted genomic DNA will then be tested for presence of the different hemoparasites using specific primers in a nPCR setting.

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Rachel is a very talented young scientist who fully immerses herself into any project she is working on. I cannot wait for her proposed project to begin and I am sure Rachel will figure out what kind of blood parasites are infecting white-tailed deer in the Shenandoah Valley.

Project Timeline

The proposed research will take 10 months to complete. Following sampling, deer parasitemia will be briefly assessed using Giemsa staining and light microscopy. Genomic DNA will then be isolated from blood spots collected on filter paper. The extracted DNA will be used to perform nested PCR (nPCR) assays with species-specific primers targeting the different parasite species. The presence of each parasite species will be further confirmed by sequencing.

Jan 22, 2026

Project Launch

Feb 04, 2026

Project Launched

Mar 31, 2026

Sampling (Completed)

Apr 20, 2026

Parasitemia

Jun 01, 2026

Genomic DNA Extraction

Meet the Team

Rachel Olive
Rachel Olive
Post-Baccalaureate Researcher

Affiliates

California State University, Chico
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Rachel Olive

I am a recent graduate in microbiology from California State University, Chico, with a bachelor's degree. I am engaging in post-baccalaureate research at CSU Chico while applying to Chico's Biological Sciences master's program. I've always had an interest in the intersection of lab science and field science. I graduated from CSU Chico with my bachelor's degree in Microbiology in the fall of 2025. During my undergraduate studies, I received extensive exposure to various fields of science through all my classes. I learned valuable lab skills, how to collect samples in the field, how to culture bacteria, and how to understand research papers. In the spring of 2025, I took hematology, and I knew that was the field that I wanted to pursue.

For the last year, I have had the privilege of working on a deer malaria project in the laboratory of Dr. Nicole Kilian at California State University, Chico. I joined the project under the guidance of another student and eventually took it over upon that student's subsequent graduation. In this research, I have been able to refine my laboratory skills and engage in field work, collecting samples and analyzing them in the lab.

Now that I have graduated with my Bachelor's I am looking to utilize this data to provide the groundwork for a master's thesis, should I be accepted to Chico's master's program in the fall. I believe that this project has the potential to reveal so much about the hemoparasitic activity in white-tailed deer residing in the Shenandoah Valley.

Lab Notes

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