This will be the first study to provide data regarding GI permeability, inflammation, and endotoxin translocation following resistance exercise and to compare resistance exercise induced GI disturbances between men and women. We will seek to utilize fecal shotgun meta-genomics and metabolite analysis to determine if the gut microbiome or short chained fatty acid (SCFA) concentrations are associated to resistance exercise induced GI distress and whether gender specific differences are present.