Pure cultures growing on sludge and forest floor debris
We have accomplished maintaining pure cultures of Mycena cinerella that grow on sterilized forest soil and sterilized forest floor organic debris that had been saturated with toxic sewage sludge. The next step will be to induce the fungal mycelium to produce new mushrooms on the contaminated forest soil and forest floor debris materials. The mycelium also grows well alone in flasks of liquid medium, which is important for analysis of the fungal culture medium extracts for the presence of novel antimicrobial agents the fungus is anticipated to produce in response to exposure to various concentrations of fractions of the toxic sewage sludge leachates.
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