Lyme disease is the most commonly reported vector borne disease in the United States with over 300,000 human cases reported annually. While the white footed mouse, Peromyscus leucopus, is the most common reservoir of the disease, Lyme is present outside of the range of this species and in habitats it does not use. The goal of this study is to identify what species serve as reservoirs of Lyme disease in restored prairie ecosystems and how common the disease is in these ecosystems.