Aperiomics is a company that provides Next-generation DNA and RNA sequencing to detect pathogens from samples, including humans and insects. From one sample, every pathogen can be identified in one test. The proposed project will adapt our existing technology for detection of Lyme Disease, as well as other pathogens from ticks. This test will be adapted to human testing in subsequent phases. The ultimate goal is a rapid, robust test for Lyme in all sample types.