The majestic tiger is an iconic resident of zoos, fascinating people of all cultures. This project will determine what is going on inside tigers. The tens of trillions of microbes found in a gut microbiome influence many aspects of health, and yet are unseen and unknown to us. Using non-invasive sampling methods, we will identify the microbial zoo of the tiger gut, with the ultimate aim of better understanding how to promote a healthy microbiota that will ensure healthy tigers.