In 1895, Daniel O’Connor released three Australian lungfish into a river outside the species’ known range. The Pine River has been considered an introduced population ever since. New evidence raises some doubt about this assumption - maybe they were there all along? If so, preservation of genetic stocks in the Pine River may be vital for conservation. We will clarify the status of Pine River "lungies" as resident or introduced using a new Sequencing By Genotyping method.