Thomas Irvine

Thomas Irvine

University of California at Davis

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Haven't created any projects yet! 

Thanks for donation. Of course it helps!
As some of you may have noticed, I have lowered the desired amount of funding. I have done this for three reasons: 1) The costs of the CRISPR system have dropped by a factor of 10, due to non-profit plasmid sharing. 2) Sequencing prices at UC Davis dropped by 30% 3) A Post-Doc in my lab has o...more
From the bottom of my heart, Thank you Chris. You are helping to keep my dream alive.
Abdullah, At this point, I am not planning on using a paired nickase. I want the payload to be as small as possible to ensure adequate and efficient dispersal of the viral particles. I know I'm not answering your question fully. This is a crowd-sourcing site for experiments that are not fully...more
I have a standardized version of the CRISPR plasmid that I produced using a technique developed in my lab. I cannot divulge the details, as this would not be fair to the others in my lab who developed the technique.
Thank you for being the first person to show interest in my project. Yeah the idea is to attack the genetic element added by Agrobacteria with the expressed CRISPR/Cas product. We will only be using one bacterial culture at a time to utilize a single type of plasmid.