Thomas Irvine

Thomas Irvine

University of California at Davis

More

Haven't created any projects yet! 

Thanks for donation. Of course it helps!
Sep 17, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment
As some of you may have noticed, I have lowered the desired amount of funding. I have done this for three reasons: 1) The costs of the CRISPR system have dropped by a factor of 10, due to non-profit plasmid sharing. 2) Sequencing prices at UC Davis dropped by 30% 3) A Post-Doc in my lab has offered to give me a lot of the materials I require because he recently finished a CRISPR experiment in rice and no longer requires them. I will supplement any other costs, outside of my funding goal, with money out of my own pocket.
Sep 11, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment
From the bottom of my heart, Thank you Chris. You are helping to keep my dream alive.
Sep 08, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment
Abdullah, At this point, I am not planning on using a paired nickase. I want the payload to be as small as possible to ensure adequate and efficient dispersal of the viral particles. I know I'm not answering your question fully. This is a crowd-sourcing site for experiments that are not fully off the ground, therefore I cannot divulge too much at this point. This is not a grant proposal so I have to be wary about releasing too many details. I have already seen some other experiment pages that have used similar information to what I have posted. Some of my methods must remain proprietary until I publish, and so I have intentionally been scientifically vague or miss leading in certain places. All your answers will be revealed when I publish this open source. Thank you for your interest! If you are really interested, feel free to donate. I will be able to give you the answers you seek sooner! Thomas I.
Sep 07, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment
I have a standardized version of the CRISPR plasmid that I produced using a technique developed in my lab. I cannot divulge the details, as this would not be fair to the others in my lab who developed the technique.
Aug 18, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment
Thank you for being the first person to show interest in my project. Yeah the idea is to attack the genetic element added by Agrobacteria with the expressed CRISPR/Cas product. We will only be using one bacterial culture at a time to utilize a single type of plasmid.
Aug 18, 2014
Can accurate DNA editing stop the formation and growth of tumors?
View comment