chowe2

I am an independent early-career researcher with a background in bioengineering. I have gained my skills and experience as a wet lab and dry lab researcher while having worked on a wide variety of molecular and computational biology research projects. The most relevant of those projects include in-vitro mutagenesis library screening of enhanced fluorescent and luminescent proteins, development of programmable genetic switches in E.coli for the iGEM synthetic biology competition, in-silico affinity maturation of antibodies, and de novo design of novel CD20 binders with RF Diffusion. My introduction to carbonic anhydrase (CA) protein engineering occurred during my time at a climate biotech startup, where I was tasked with a deep literature search on state-of-the-art CO2 capture biotechnologies and learned about how useful CA is as a biocatalyst in large-scale CO2 capture technologies. While I have carried out mutagenesis assays and designed de novo proteins in-silico, I have not yet worked with CA’s before and have not engineered proteins for thermostability. Overtime I have built out a molecular biology lab where I can carry out small-scale experiments independently. For this project, I plan to outsource all synthesis and cloning steps, while taking on the in-silico design, the CA assay of variants, and the small scale in-vivo continuous directed evolution steps myself for ultrastable CA discovery.

LinkedIn: https://www.linkedin.com/in/coreyhowe/

September 2023