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Here we see a gel filtration result of purified NPC1. The large peak contains NPC1 and other proteins that are potential interactors. This is one way we try to tease out complexes.T...
Cholesterol is released in lysosomes from LDL particles through the action of acid lipase. NPC2 protein carries the free cholesterol to NPC1 where NPC1 takes part in getting cholest...
Here is one of our first gels that shows we can pull down NPC1 interacting proteins. NPC1 is the thick dark band and other bands are unknown proteins. We are still in the process of...