rgrogan

Are you going to include fluoride in your trace minerals? There seems to be a dearth of data (like https://getd.libs.uga.edu/pdfs/bloodgood_jennifer_c_201612_phd.pdf introduced F but then no info). Fluoride is in most seafoods, and is in sea turtle chow, apparently from the natural fish and seaweed meals (https://pims.purinamills.com/BusinessLink/media/Mazuri/ProductSheet/5B37.pdf?ext=.pdf ). Fluoride could be studied to see if it affects organ development. (Glenn ASDC Journal of Dentistry for Children 1984;51:19-23. A common developmental defect called pits and fissures is reduced from 28% to 0% in these organs.) Or perhaps the timing of some developmental milestones. A good baseline nutritional study would be a good start.

Thanks for taking on a very difficult but important project. Maybe maybeI Don Ingber's team at Wyss could make you an organ-on-a-chip that could secrete your protein into a solution in which it would not fold up. Then you could inject changes into the solution - polarity, etc. and "watch" it move.

Not that you need any more variables to keep track of at this stage, but have you looked for effects on the next generation? For example, in humans the nutrient folic acid seems to prevent birth defects if taken in the first critical weeks of pregnancy. There are about 15 nutrients that may have similar effects. So maybe in your cool fish something the parents take could affect the lifespan of their offspring. Anyway, great experiments, logic, and pix. Holler if you want a Hawaiian taro plant for your aquarium.