Robert Kuhn

We have around 50 samples that had poor amplification for CO1, so a summer project has been re-extracting DNA using a new kit (Qiagen DNeasy Blood and Tissue) from the remaining pillbug soft tissue...

As a summer project I will grow non-Rj2 variety Tankuro soybeans in seed starter and inoculate with Bradyrhizobium japonicum after about 10 days. This way we can harvest nodules, grow up the Rhizob...

We have worked through most of our pillbug samples, extracting DNA and doing PCR for CO1 mitochondrial haplotypes and SRDs through 4 primer sets. It has been a lot of work on a school schedule but ...

First of all, this is a project for high school biotechnology students. In designing the project I wanted to pick an underused biotechnology model in schools (plants), a cool and well known symbios...

After some issues with primers we are back on track. Each pillbug sample will have 5 PCR runs. That's a lot of PCR. From these, three potentially can be sent for sequencing. The COI will be sent fo...

We have successfully extracted DNA from over 100 pillbugs submitted from Georgia, North Carolina, California and New York. Each sample will have PCR and gene sequence of both the COI mitochondrial ...