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Are current antibacterial treatments resulting in the evolution of more virulent multidrug resistant bacteria? Garcia-Contreras, Rodolfo, and Jesús Guillermo Jiménez-Cortés.. UNAM, 24 Dec 2017. Experiment. doi: 10.18258/10486
Bacterial cultures, growth curves, minimal inhibitory concentration determination, efflux pumps assays, quorum sensing evaluation via determination of signal molecules with reporter strains and chromatographic methods, determination of phenazines and siderophores (by spectrophotometry), exoprotease activities, exopolysaccharide, rhamnolipids, swarming, biofilm formation, etc.
Galleria mellonella infection model (larvae are infected by the injection of bacteria in the hemolymph and survival is evaluated daily).
The main challenge will be to obtain enough funding.
We will use standard statistical methods to analyze our data, biology is noisy, but having a high number of replicate experiments help.
This project has not yet shared any protocols.