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CRISPR Capsules: Packaging Cas9 with bacterial outer membrane vesicles

By Northwestern IGEM, Jordan Harrison, Shu Huang, Sam Davidson, Tasfia Azim, User12121, Tyler Lazar, Paul Perkovich, and Sara Boyle
Backed by Michael Geer, Irv Kuperman, Geraldine Piglowski, Emily Feldman, Karen Levick Boyle, Robert Kennedy, Jacob Roth, Qiu Ling, Jamie Davidson, Diana Tang, and 37 other backersSylvi Kuperman, Tim Sheng, Ailey Adorjan, Xiaogang Shen, M. M. Azim, Deirdre And Jack Lazar, Nathaniel Putnam, Richard Ozer, Tony Wenyuan Huang, Betsy Padgett Villanueva, Yasminda C, Aakash Gandhi, David Camposeco, William Grunow, Carolyn Kreines Kuperman, Steven Yue, Sherry Davidson, Purdue IGEM, Hangda Zhang, Denny Luan, Jimofoz, Marty Kellams Yochum, Cara Madansky, Lucia Brunel, WDD, Erik Barillari, Matthew Lam, Mandy Liu, Ricardo Perez, Alexander Hoekstra, Matthew Jack, Thane Thompson, Julie Matsubara, Cindy Wu, Christina Tran, Kate Ray, and Eric D. Walters
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Northwestern University
Evanston, Illinois
BiologyEngineering
DOI: 10.18258/7292
$2,014
Raised of $2,000 Goal
100%
Funded on 7/27/16
Successfully Funded
  • $2,014
    pledged
  • 100%
    funded
  • Funded
    on 7/27/16
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  • Overview
  • Methods
  • Lab Notes (11)
  • Discussion (11)

Lab Notes

Tags:

experiment designOMVvesiculationprocedureCRISPRCas9antibiotic resistancesuperbugouter membrane vesiclegRNAconstructsexperimentsprotein expressionGibson assemblyWestern blothypervesiculationMatt DeLisaprocessupdateiGEM 2016sequencingoutreachCTDplates

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That's a wrap!
April 21, 2017
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Emerging from Our Update Hiatus
September 14, 2016
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Updates!
August 17, 2016
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Thank you backers!!!
July 26, 2016
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Three Days Left to Become a Jedi of Science
July 24, 2016
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What the heck is iGEM anyway?
July 19, 2016
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How Can We Defeat Superbugs with CRISPR/Cas9?
July 14, 2016
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More bacteria art
July 12, 2016
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Churning Out OMVs
July 12, 2016
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Ordered DNA!
July 8, 2016
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Outreach
July 8, 2016
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