The Quick Extract method for DNA isolation
For PCR methods where you want an answer fast, and not necessarily a quantitative answer, the quick extract method is convenient. This can alternatively be made out of chaotropic salts. For these kits, however, we bought a quick extraction buffer at a reasonable price. (Methods for making your own buffer will be included in appendices).
Items in the kit
pre-labelled tubes with Quick extraction buffer (tubes labelled QE-"").
Sterile Q-tips
Equipment you will need for the extraction:
Heat block or Water bath set to 65degreesC
Steps:
1. take 3 sample tubes labelled QE-CS, QE-Sus1, QE-Sus2
CS = crime scene, Sus1 = Suspect 1, Sus2 = Suspect 2
Note that are going to develop other Mysteries - some including plant materials, such as wheat, barley, rye DNA for detecting gluten in a "crime scene" for a mystery of "who got glutened and which food did it?" . This may be important as not everyone will be comfortable working with pig or cow samples.
2. using a the sterile q-tips, swab the "crime scene" and place swab into the tube labelled QE-CS.
Crime scene = beans with bits of fur on the beans.
3. using a the sterile q-tips, swab the "suspect 1" and place swab into the tube labelled QE-Sus1.
Suspect 1 (either fur will be attached from grooming local park cow or a stand-in of a piece of beef steak)
4. using a the sterile q-tips, swab the "suspect 2" and place swab into the tube labelled QE-Sus2.
Suspect 2 (sample will be either fur from grooming local park pig or a stand-in of a piece of pork)
5. Move each swab around vigorously in each tube for about 2 minutes.
6. Close the cap and place in the Heat Block or water batch which should be at 65degreesC, for 5 minutes.
7. Place on ice.
These are your DNA samples.
- Published on Jun 16, 2024
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