About This Project
Hybridization of a native species with an exotic species can pose threats to the natives through homogenization/replacement of the native genotypes. The native Red Mulberry (Morus rubra) and the exotic White Mulberry (M. alba) co-occur in eastern North America, both species are reported to undergo hybridization. Through the genetic fingerprinting, we will find out whether hybridization occurs and determine how it affects the integrity of the native Red Mulberry.
Ask the Scientists
Join The DiscussionWhat is the context of this research?
Previous studies on the two species (inferring hybridization) suffered from taxonomic misconceptions and lacked rigorous genotyping data. This project is built up on our recent works on taxonomy, phylogeny, breeding system and sex ratio studies. Since the identification of several varieties of M. alba hybridizing with the native M. rubra is very challenging, a more rigorous genotyping study with wider sampling is warranted. The proposed project will also generate preliminary data for a grant application to be submitted to the National Science Foundation. The outcomes will have implication in the conservation of the native Red Mulberry.
What is the significance of this project?
There is a great need for work that generates evidence that the invasive white mulberry is posing threats to the integrity of native species through genetic swamping. We will also highlight some taxonomic confusion even among some experts and general public. This we hope will create a movement campaigning against the invasive species and conserve the native species.
What are the goals of the project?
In the spring and early summer of 2017, we will conduct field studies for taxonomic identification, collect herbarium samples for voucher preparation, and collect fresh leaf samples in silica gel for the DNA work. In the fall of 2017, the DNA samples collected from 15 individuals from 12 populations will be subjected to Polymerase Chain Reactions using 15 microsatellite primer pairs, and subsequent fragment analyses.
This will provide preliminary data for a competitive grant application to be submitted to federal funding agency. The results can be used as an example evidence on how hybridization can cause taxonomic issues and induce invasiveness of the exotic species.
Budget
One MS graduate student will be recruited as a graduate teaching assistant supported by the Department of Biology and Microbiology at South Dakota State University. (Each faculty gets one GTA support from the department).
The budget requested will cover travel expenses for field trip for sample collections. The travel expenses is at the lowest minimum because I have colleagues willing to collect samples, and send from their local areas in the eastern and southern United States. In addition the requested fund will allow us to cover expenses for lab supplies and contractual for microsatellite genotyping and sequencing of two species-specific DNA markers.
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Additional Information
Project Backers
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- $10Total Donations
- $5.00Average Donation