Methods
Summary
Cultivation of rice will be conducted in pots. There will be two CO2 levels that will be used to treat the rice, namely ambient (400ppm) and elevated level (800 ppm). Treatment will be conducted using solar plant factory facility equipped with controls and sensors to monitor microclimate. Leaf photosynthesis will be measured at maximum tillering and at harvest stages. The plant growth will be monitored by observing agronomic traits (height, tiller number) and yield will be harvested at the end of the experiment. Leaf, stem and root samples will be obtained from the rice plants during the measurement of photosynthesis to determine the morphology and anatomy and identify the changes brought by the CO2 treatments. At harvest, grain quality will be determined by measuring the physical grain properties and chalkiness.
Pre Analysis Plan
For all the data from the traits that will be collected, the data will be analyzed by ANOVA. Comparison of means of each trait for each variety will be analyzed. PCA will be used to identify groups of traits that showed variation at the same time and correlation of traits will also be analyzed. Path coefficient analysis will be conducted to identify the traits directly contributing to higher yield at elevated CO2 concentrations.
Protocols
Browse the protocols that are part of the experimental methods.