Methods
Summary
Methods and Procedures
3 volunteers will have blood drawn by a certified phlebotomist at the following timepoints
8 PM: Baseline
8 AM: 12 hour fast
4 PM: 20 hour fast
8 PM: 24 hour fast
9 PM: 30 minutes after refeeding
An ELISA analysis will be performed to quantify the presence of IGF-1, mTORC1, and Klotho protein in each volunteer at each time point. The resultant data will allow us to create expression curves for each protein over a 24 hour fast and refeeding.
Protocols
This project has not yet shared any protocols.