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The Generation of a Recombinant Vaccine for Bourbon Virus Furneaux (Jnr) , Henry.. , 14 Dec 2016. Experiment
I will examine the sequence of the Bourbon virus envelope protein using the antigenicity bioinformatic algorithm.
This algorithm examines the basic properties of the amino acid
sequence and predicts whether they will be seen by the immune system.
have chosen the envelope protein because it is on the outside of the
virus and accessible to the immune system. Most viral vaccines are based
on envelope proteins.
The DNA sequence of the segments that are suggested to be highly
antigenic, will be synthesized and subcloned into bacterial fusion protein expression vectors.
The fusion protein will be induced, extracted and purified. The resulting purified proteins ( mixed with adjuvant ) will be injected into mice to
determine the segments with the highest antigenicity. Antigenicity
will be assayed by western blot for antibodies to the original
The most likely challenge is that none of the purified proteins will generate a significant immune response. If so, we will seek alternative vaccination strategies, including generating fusions with TLR proteins and trying to use a mRNA based vaccine.
We will examine the titer of the immune response to the purified envelope proteins. We suspect that high titers ( > 1/20,000) will identify the envelope protein vaccines that will protect against Bourbon virus infection. At this point, we will seek a collaboration with an academic research lab to test the efficacy of the vaccine to protect against Bourbon virus infection.
This project has not yet shared any protocols.