From BUBlood to Bubenome - Part 2: Reading BUB's DNA sequence: the raw data

Jan 18, 2016

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From BUBlood to Bubenome - Part 2: Reading BUB's DNA sequence: the raw dataHi again,
Today we're back with a new episode of the videolog, where I'll explain what BUB's DNA sequence looked like fresh off the sequencing machine. A couple of things to note: as our videos will get more and more technical please feel free to leave comments or to contact us, if you'd like us to clarify anything. Also, since we can't include all the details, we'll start leaving links to tools and papers together with the video, so you can follow up on stuff if you're interested. Hope you enjoy!

More info about:
 - the fastq format on wikipedia 
- a technical note by Illumina about understanding quality scores
- a table for converting the characters in fastq files into an actual quality score

Denny, Ravaux, and 4 others like this.

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About This Project

The LilBubome - Sequencing LilBub's Magical Genome

The LilBub Genome Collective

LilBub is a magical critter: she's a lovely cat with an extraordinary appearance. Her special traits are caused by alterations in her DNA. We want to find the cause of LilBub's appearance: we will sequence LilBub's genome and look for variants that could explain her unique looks. We will closely document every step of the process, from crowdfunding to experiments and analysis, and explain what we're doing as we're doing it.