Adrian Filips

Adrian Filips

Jun 05, 2023

Group 6 Copy 33
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Extended use

Besides storage, the device can also used for short term transportation and workbench top storage during the duration of an experiment. So we will extend the testing scope to include these uses.

At this point we are considering testing by using a procedure were a known DNA sequence containing a specific enzyme cut site will be cut with the enzyme and the result ran on a gel. If the enzyme is very active the band size for the specific fragment will be larger and darker. We will choose several enzymes known for their sensitivity to temperature.

For bacteria storage testing we will store GFP expressing bacteria and we will measure the fluorescence of stabs stored or transported.

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About This Project

Temperature-sensitive reagents are common in biology labs, and often
need to be stored in expensive ultra low temperature ULT refrigerators.
This is one of the highest cost barriers to setting up a biology lab and
makes research incredibly difficult in low resource settings. An enzyme
cooler stored within a standard freezer can be a viable alternative. We
plan to design, build, and test an open-source enzyme cooler that can be
built for as little as $25 without sacrificing performance.

Blast off!

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