Yuriy Fazylov

Yuriy Fazylov

United States

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"CRISPR experiment involving gene editing to determine the maternal to zygotic transition in rice" details? were you going to simply cut out the genes or repress them somehow?
Oct 28, 2014
Can accurate DNA editing stop the formation and growth of tumors?
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This is just an assumption. With all the medical application of either technology your aspirations seem to be therapeutic rather than plant based. I don’t understand why not just explain that you can’t work with animal cells at current time and that plants are the next best thing. Why draw parallels? Literally cuts a virus in half, huh? – I would assume that more than one sgRNA is assimilated into the CRISPR locus. It’s not just a “Cas” but Cas9. I think you used “humanity” wrong. join https://groups.google.com/forum/?utm_source=digest&utm_medium=email/#!forum/crispr/topics -Just jealous
Sep 12, 2014
Can accurate DNA editing stop the formation and growth of tumors?
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let me know what you find
Aug 22, 2014
Living Skin Therapeutics
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Excuse my curiosity. I didn't realize I hit intellectual property questions so soon.
Aug 18, 2014
Can accurate DNA editing stop the formation and growth of tumors?
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No problem. I was interested in using CRISPR-Cas9 on plants myself, mostly as recombinant Cas9. What kind of guide RNA will you have? sgRNA or crRNA + tracrRNA? Have you tried looking into; if the Ti vector already has a spacer associated with it in agro or other bacteria that speedily does away with it? What type of Cas9? dCas9-FokI? What is your PAM target? What is your CRISPR/Cas9 delivery method?
Aug 18, 2014
Can accurate DNA editing stop the formation and growth of tumors?
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what agro and Ti vectors will you be using? wt or lab cultured? how will you know if there is a diversity in Ti vectors? what is your gRNA target? T-DNA itself?
Aug 18, 2014
Can accurate DNA editing stop the formation and growth of tumors?
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Having read up on melanin and its enzyme polyphenol oxidase, I got the gist that in bacteria the enzyme’s metabolites interfere with cytochrome c activity. Could it actually hinder your engineered cells? What is melA cell localization?
Aug 15, 2014
Living Skin Therapeutics
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holy cow. you just gave me an idea.
Aug 12, 2014
Living Skin Therapeutics
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Having read up on melanin and its enzyme phenol oxidase, I got the gist that in bacteria the enzyme’s metabolites interfere with cytochrome c activity. Could it actually hinder your engineered cells? What type of melanin are you aiming to produce? What is its cell localization?
Aug 12, 2014
Living Skin Therapeutics
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Thank you. Your will be appreciated.
Aug 11, 2014
Can we make radiation-resistant plants that can survive in space?
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I don't like not knowing. http://cosmiclog.nbcnews.com/_news/2007/01/05/4350893-lost-cities-seen-from-space http://www.pbs.org/wgbh/nova/ancient/maya.html CaCO3 seems to be associated with the answer to your question. Look for a plant that would allow for high amount of Calcium to buildup.
Aug 11, 2014
Can we make radiation-resistant plants that can survive in space?
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>A crazy idea. But why not use Cyan Fluorescent Protein instead of melanin? Yes crazy idea, mainly because of limitations in stokes shift activity of CFP. It gets excited not even at long, UV-A, but violet 435 nm - and emits back at blue 485 nm. Those are its peak activities. It doesn’t do a very efficient job at it. It doesn’t mitigate all of the other frequencies that would ionize cellular content. 435 nm is nothing to a plant compared to let’s say UV-C. I’m trying to find a mechanism that would deter all UV and more. Eumelanin seems to be the polymer for the job. The aim of this project is much more Gamma, X-ray, UV-light encompassing. It is trying to discover a way to protect the cell’s contents; proteins, DNA, etc. There is no difference in how much light you shine onto or within a contemporary plant. Its light harvesting mechanisms can only take so much before the rest of it seeps through. Or gets red-shifted via pigments plants produce to protect themselves. I’m curious. There are dozens of FPs out there. What made you choose cyan?
Jul 28, 2014
Can we make radiation-resistant plants that can survive in space?
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