Annotation Update
No doubt we lost some time with the whole COVID-19 mess and the shutdowns; but we're through the RepeatModeler and RepeatMasker is finishing up; unmasked BUSCO complete genes are at 55%, which is p...
QC'ing & Diploid aware assemblies
QC'ing & Diploid aware assembliesWe're moving through "playing", or rather evaluating assembly parameters for the haploid version and assesing the quality of the assembly by comparing and contr...
Assembly Complete; onto polishing and BUSCO QC'ing
Using two SMRT cells (PacBio SII) worth of long-read data (N50 > 32,000 bp) we opted to use the wtdbg2 redbean (https://github.com/ruanjue/wtdbg2) assembler, we have assembled the wood frog geno...
First Assembly is Done!
First assembly of the wood frog genome is done! 8.6 G Bp - that's 2.5x the size of the human genome. We've done the initial sequencing run using the minION nanopore sequencer that produces ultra ...
Interesting XPrize - Consider Voting for Cryopreservation
Here's a opportunity to vote for an upcoming "Grand Challenge" for the XPrize: http://www.xprize.org/prizes/future-prizes/cryopreservation#.VVEhEG1euyc.facebook
Good News / Bad News (not really)
Just wanted to give everyone a heads up on a new development that's just come about. The company that sells/sold the kit (Illumina) has just announced a new iteration of their DNA sequencing machi...
Update - WF Genome
Hello, We have obtained the kit from Illumina and are in the process of building the sequencing library. I have also recruited three undergraduates to help with the annotation. We hope to genera...
Thanks!
Hello, Thanks to everyone that pledged! With help from Illumina and all of you we hit our goal! I'll be keeping everyone posted on the project.Regards,Andor
Lower Budget!
Hi Everyone, Some good news - I've been working with Illumina and they've been able to lower the cost of the reagents by $750 - that brings our overall budget down to $3000! AND much closer to o...