Chris Rehberg

Chris Rehberg

Sydney, NSW, Australia

Amatuer Naturalist, IT specialist, Photographer

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Published on Dec 25, 2017

Light micrographs published!

Ok, so I did say the results would be out before Christmas. It's Boxing Day here in Australia, but I've still got half an hour of Christmas Day left in the UK, five and a half hours in New York and...

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Published on Nov 18, 2017

Light microscopy results almost ready...

Hi Everyone!Ok - last time I said the next update would be the link to the results. They'll take me a little longer, so apologies for the delay, but here's the update on what's been happening and w...

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Published on Oct 22, 2017

Light results coming soon

Hi Everyone,Here is a quick photo of all the hair types, sitting on slides -Over hair at the front, under hair at the back, and guard hairs all in between.I recently received a measurement slide - ...

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Published on Oct 08, 2017

Light microscope images

Hello Everyone,A quick update to advise that most of the light microscope images have been taken -Four slides were prepared. The first contained two under hair fibres; the second contained two guar...

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Published on Aug 10, 2017

All components arrived

Hi Everyone,A quick note to update you that all pieces of the puzzle have arrived.As noted earlier, the CSIRO made a generous offer to conduct high resolution scans for this project. That resulted ...

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Published on Jun 22, 2017

Microscope arrived - and new CSIRO images

Two new updates to share with everyone.Firstly, the microscope has arrived! We're a little bit behind schedule, but I've had it set up for almost a week now. During this time I've been preparing te...

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Published on May 05, 2017

Making news, expanding scope, last day to crowdfund!

Hello everyone!Just 24 hours until the crowdfunding project closes - and ~$700 to target! - still time to spread the word and get funding over the line!It has been a busy week in the realm of Tasma...

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Published on Apr 17, 2017

Collating reseach papers - prospective technique for automated species identification

Over the past two weeks I have been collating research papers and information on the following:* procedures for examining hair under the microscope* procedures for preparing whole mount hair slides...

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Hi Denny, The light microscope photos will look different. They will show less detail because only a small fraction of the hair fibre will be in focus at any given time. I plan on creating a video that shows the focal plane move through the depth of the fibre as I adjust focus. The point of this is to provide a reference that others can use who have access to a light microscope. The SEM and CLSM both show more detail and full focus (and higher magnification, in some images). These higher resolution images bear out the illustrations in the 1950 paper, but for anyone relying on a light microscope, they still have to match what they see to the species. Having light microscope images alongside SEM may help people interpret what they see under light microscopy. (And of course, the SEM and CLSM will help anyone else using SEM or CLSM to verify an ID on a field sample - if such a sample makes its way to one of those machines). The sample I have is a small envelope with several hairs in it. I will use different individual hair fibres for the light microscopy than the ones used in the SEM / CLSM.
Jun 24, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Thanks Brian, me too! If you haven't already, check out the latest lab notes - CSIRO has made an offer to add scanning electron micrography to the mix and images are now online here: http://www.wherelightmeetsdark.com.au/research/tasmanian-tiger-(thylacine)-research/photomicrographs-of-thylacine-hair/sem-scanning-electron-micrographs-of-tasmanian-tiger-hair/
May 18, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Hi Meika, Thank you for your commitment on your friends' behalf! :D
May 06, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Hi Tina, Thank you for your encouragement! Getting out there is one part of searching, but this hair project will be an invaluable tool for everyone - already people have started contacting me about hair they've collected on various projects and they're looking forward to comparing their own samples with the images to come through this project. Thank you for your support!
May 06, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Hi Brian, thank you for your support! The target has been reached today and just a few more hours until the deadline! Can't wait to get started and get some great images out for everyone to use.
May 06, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Hi John, thanks for the heads-up. I'm actually having difficulty playing back the video via the Experiment website and have let the staff there know. I will see if I can take a look via a different computer/browser later today. Can you tell me what important information was left out? For anyone else wanting the direct YouTube link to the video, it's at: https://www.youtube.com/watch?v=MOErQdNfQxA
Apr 09, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Awesome - will be really cool to get photos like these out there. Thanks so much for your pledge!
Apr 07, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Hi Jon - would love to try and ID tiger hair. There are a *lot* of other mammals to learn first too! However a colleague who has collected hair from nest/bedding material in a cave is already lining up to send it to me once I get these reference pictures done. Thanks for your pledge!
Apr 07, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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Let me ask the first question: Have you considered leasing microscope time? Answer: Yes. My nearest university with microscope leasing for external researchers is the University of Sydney. Being external to the university and not a publicly funded research organisation means that I will be liable for commercial rates (see link at bottom). There is an A$270 registration fee, followed by hourly rates of A$345. Completing this project will require preparing sample slides using non-thylacine hair before moving on to the much scarcer and more valuable thylacine samples. I imagine I will spend time preparing a set of non-thylacine samples, learn that there may be a better approach - in slide prep, lighting, composition, image capture, etc - then re-do the non-thylacine samples until technique and approach are all adequate for the final result. At this point I will then progress to the thylacine hair. At an incredibly conservative 4 hour estimate, the cost of leasing would total A$1,650. It's at this point that I think funding a purchase becomes financially viable. In reality this work will take many days of microscope time, and weeks of actual time to complete. In addition, a dedicated scope can be set up and remain ready for use - there is no repeated set up and tear down time. There is no need for travel time to the university each time I am ready to continue the slide work and there is no need to transport the valuable thylacine samples and risk their loss or damage in transit. USyd leasing fee schedule: http://sydney.edu.au/acmm/pdf_doc/SMM_Fee%20Schedule_2017.pdf
Mar 28, 2017
Micrographic study of thylacine hair (Thylacinus cynocephalus)
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