About This Project
Indonesia is one of the largest palm oil producer in the world. Palm oil waste such as oil palm empty fruit bunches are polluting our country. We try to degrade oil palm empty fruit bunches into useful products such as bioethanol and cellulose acetate using bacterial cellulose from intestinal wastes slaughterhouses. we make a recombinant bacterium capable of degrading cellulose palm oil Empty Fruit Bunch. The benefits of this research is to reduce waste pollution and produce a useful product.
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What is the context of this research?
One palm oil waste in the form of solid biomass lots is empty fruit bunches (20% of the total weight of whole). The composition of alpha cellulose is significant in empty oil fruit palm bunches is potential to be used as raw material for the manufacture of cellulose acetate which is an organic ester important that used in textiles, cigarette filters, as well as materials membrane maker. other than that the composition of the cellulose can be converted into bioethanol with the help of bacterial cellulases. In this study, we isolate bacterial cellulases as Ruminococcus sp. waste from slaughterhouses intestine, then the gene encoding the corresponding be inserted into Escherichia coli. The result of the insertion into recombinant bacteria capable of degrading oil palm empty fruit bunches.
What is the significance of this project?
This research is useful to give way in the processing of waste from palm oil industry and slaughterhouse waste, generating recombinant bacteria capable of degrading oil palm empty bunches effectively, then becomes a more useful product. problem to be solved is how to produce the type of recombinant bacteria capable of degrading oil palm empty bunches as long as these bacteria work in symbiosis. Here we tried to use the principle of synthetic Biology.
What are the goals of the project?
produce recombinant bacteria capable of degrading waste cellulase independently. We started the research, as April 2016.
Our research design are:
1. isolation of bacteria from the intestinal waste
2. characterization and identification of bacteria Ruminococcus.
3. Decision cellulase gene coding
4. The insertion of the gene into E. coli
5. Test for cellulose degradation in oil palm empty fruit bunches.
This budget is very helpful for our research and follow a competition called IGEM. we not have sufficient sponsorship to help implement this project. besides, we had trouble in the introduction of research products to the public due to lack of funds.
Meet the Team
Starting with IGN-TTRC as the consortium to improve teaching, training and research collaborations through mutual trust and support within the partners universities. Many activities already hold with the main activity was teaching and training that divided into five modules for five years at two different universities, Brawijaya University and Andalas University. One of the aims for this consortium is to designed and established long term scientific cooperation between Indonesian partners as well as between Indonesian and the University of Kassel. As the follow up of that aim, the consortium tries to develop and deepen the collaboration through larger scope of activity especially focusing on researches collaboration. The partners universities is realized that the networking must not stop until the five module completed but need to deepen and keep in more flexible format with the Indonesian – Germany Networking as the basic fundamental. With this concept, hopefully it will create more fascinating activities that bring a lot of advantages.
Till now, there is some changes in the consortium members with several universities enter as a new member. The consortium currently consists of the following members:
- Univeritat Kassel, represent by Prof. Dr. Nellen, Gunter Backes
- Brawijaya University, represent by Prof. Fatchiyah
- Andalas University, represent by Prof. Ari Jamsari
- Mulawarman University, represent by Prof. Krishna Purnawan Candra
- Sam Ratualangi University, represent by Dr. Trina Tallei
- Jember University, represent by Dr. Kartika Senjarini
- Airlangga University, represent by Dr. Bambang Irawan
- Gadjah Mada University, represent by Dr. Yekti Asih Purwestri
- Jend. Soedirman University, represent by Condro Wibowo Ph.D.
- State University of Malang, represent by Sri Rahayu Lestari, M.Si.
- Ma Chung University, represent by Tatas Hardo P. B., Ph.D
- Universitas Al Azhar Indonesia, represent by Yunus Effendi Ph.D.
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