Antonio Lamb

Antonio Lamb

San Diego, CA

B.S., Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, IndieBio 2016 Cohort

Plant Molecular Biologist

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Published on Jul 30, 2018

The reward for your contribution

I have spent several months trying to figure out the best way to recognize and thank the people who had donated to the crowdfunding experiment. I thought of several ideas in the past, such as givin...

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Published on Aug 23, 2017

Proinsulin expression was not detected, but recombinant GFP successfully expressed from MSX constructs

We were still unable to detect the expression of recombinant proinsulin in chlamydomonas from our pAsapI construct, despite the fact that the construct seemed to have successfully integrated into t...

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Published on Feb 16, 2017

The proinsulin gene was positively detected in our transformed algae using genotyping PCR

We were finally able to detect the algae proinsulin gene. We had re-cultured the emergent transformed microalgal colonies on minimal media to ensure that the colonies contained our pASapI-proinsuli...

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Published on Jan 04, 2017

Quick New Years Update

It has been an eventful 2016, to say the least. On the technical side, we were able to transform an antibiotic resistance marker successfully into microalgae (pictured below).  Unfor...

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Published on May 29, 2016

Completion of more E. coli expression work, paving the way for algal transformation work.

Last week, we reached another milestone. We were able to successfully confirm that our modular expression system (derived from the pASapI plasmid) is indeed functional, and working well in E. coli....

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Published on May 10, 2016

We are officially in the IndieBio accelerator program!

Thanks to the amazing support and enthusiasm we received from the community through the Experiment.com platform, our team was offered an incredible opportunity to join the IndieBio synthetic biolog...

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Published on Apr 21, 2016

A Successful Molecular Cloning: Overcoming Stubborn Structures

If you've followed our methods section, you'll be happy to know that we completed some important experiments on our path to making algal insulin a reality. First off, the proinsulin gene was clone...

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Published on Feb 21, 2016

Proinsulin Gene in E. coli and Construction of Microalgae Vector (Update)

It has been an incredibly busy few weeks, which is why this overdue update is a bit late. The algal DNA construct is already done but we do not know if it works, so we needed to test in bacteria fi...

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Reply to:Cindy WuCindy Wu
Ah it's okay. Sorry I just saw this but I'll also reach out to Bowen and see what he's doing :).
Reply to:Cindy WuCindy Wu
Thanks Cindy :). We hope to have more updates in the future. Hope you are doing well too :).
Reply to:Denny LuanDenny Luan
Thanks Denny :thumbsup:. Same to you
Reply to:jimofozjimofoz
We chose fish because it eats algae naturally and the dosage required to vaccinate fish is much lower. We've also identified that there is a clear market demand. We would of course consider other avenues if we are successful in this area.
Reply to:jimofozjimofoz
We chose fish because it eats algae naturally and the dosage require to vaccinate fish is much lower. We've also identified that there is a clear market demand. We would of course consider other avenues if we are successful in this area.
Reply to:jimofozjimofoz
That is interesting. We will look into it! Thanks for sharing.
Reply to:jimofozjimofoz
Hey Jim. Doing something like that would be quite difficult, if not impossible. Perhaps one could fuse algal protoplasts with cyanobacteria, but there are plenty of problems with it working... for instance, the host cell would probably recognize this bacterial "guest" as an intruder and destroy i...more
Thank you Willi! Wishing you the best as well.