Genetically Engineering Bacteria to Measure and Sequester Fluoride to Clean Our Waters

Durham, North Carolina
DOI: 10.18258/10683
Raised of $1,500 Goal
Funded on 3/26/18
Successfully Funded
  • $1,500
  • 100%
  • Funded
    on 3/26/18

About This Project

We are the ECHHS iGem team! Last year, we successfully tested a plasmid that identifies fluoride bioswitches. We won an award at iGem, the largest synthetic biology competition. This year's project will expand on our results. Our main hypothesis is if we find an effective fluoride riboswitch, than we can use it so sequester, bioremediate, and detect fluoride. Our team needs help to cover the competition registration fees & pay for lab materials.

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What is the context of this research?

Excess fluoride in water can cause severe health defects, such as dental and skeletal fluorosis (teeth and bones decay), as well as developmental and reproductive issues. Especially in poorer countries, where there's often erosion of sediments or volcanic rocks, toxic fluoride concentrations can have severe repercussions. Our team successfully designed a system, called the "Fluoride Riboswitch Regulated Chloramphenicol Acetyltransferase Operon" (CHOP), to characterize the responsiveness of fluoride riboswitches. This system allows bacteria to grow only in the presence of fluoride. It can be used to identify riboswitches with higher responsiveness to fluoride. However, the CHOP system is only the beginning. This summer, we will use the CHOP to combat excess fluoridation.

What is the significance of this project?

Fluoride is present in all bodies of water: oceans, rivers, lakes, and groundwater. Erosion of sediments or volcanic rocks contributes to higher levels of fluoride. Exposure to more than 6mg of fluoride everyday can be toxic, and lead to dental and skeletal fluorosis, which is the decay of teeth and bones. Doses of fluoride that result in 4.5mg/kg body weight can cause developmental and reproductive concerns and affect the growth as well as mental development of people.

What are the goals of the project?

Last year we successfully tested a DNA system called CHOP that we cloned into E. coli. Bacteria with the CHOP DNA system only grow on plates with fluoride. We also added a antibiotic chloramphenicol resistant gene, so only E. coli that have the CHOP DNA system successfully inserted will grow. For this year's project we plan to modify our CHOP system to bioremediate fluoride.

This summer, we will use our CHOP system to identify more effective fluoride riboswitches with a higher affinity to fluoride. From there, we can use the riboswitch to control the enzymes that metabolize fluoride. This will work to bioremediate fluoride.


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By contributing to our campaign we will include you or your company/organization on our posters, team wiki, and t-shirts. We will keep you updated on our research progress through lab notes on our Experiment page and share our final wiki with you when we go to the competition

Endorsed by

The East iGem Team will again focus on using ribo-switches to assess water quality. Last year the team successfully created a fluoride sensitive ribo-switch in bacteria to detect fluoride levels, which earned them the "Most Innovative Measurement" award at the iGem Giant Jamboree. Their goal is to continue in the efforts of improving their ribo-switch to effectively and efficiently detect other water impurities hopefully to improve water quality conditions for individuals world-wide! Their excitement and dedication is intoxicating!

Project Timeline

Our team's focus from now until March 31st is primarily fundraising so that we will be able to meet the $4500 registration requirement.

This summer, in the lab we will work to bioremediate fluoride.

Feb 14, 2018

Speak with mentor for specifics on materials necessary

Feb 24, 2018

Project Launched

Mar 31, 2018

Meet $4,500 registration fee

Apr 10, 2018

Begin contacting companies for materials/supplies and locate a lab 

Jul 01, 2018

Begin identifying more effective fluoride riboswitches using CHOP 

Meet the Team

Camille Lorie
Camille Lorie
Vice President


East Chapel Hill Highschool
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Ananth Murthy
Ananth Murthy


East Chapel Hill Highschool
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Team Bio

We are a second year iGEM team out of North Carolina. We are one of the only high school teams in North Carolina, and we hope to inspire teens in our area to join the exciting world of research! Last year we worked under the leadership of Dr.Joseph Harrison (a postdoc at UNC) at Kuhlman Lab!

Camille Lorie

Hello! I'm Camille, and I'm the vice president of our iGem team. As someone that aspires to go into medicine and eventually biological research, and competing in iGem will allow me an invaluable experience that will only grow my passion!

Ananth Murthy

Hello! I'm Ananth, and I'm the president of our iGEM team. I have a great love for synthetic biology, the medical field, and clinical research. I hope to pursue a career in the field of medicine.

Lab Notes

Nothing posted yet.

Project Backers

  • 7Backers
  • 100%Funded
  • $1,500Total Donations
  • $214.29Average Donation
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