This experiment is part of the Wildlife Disease Association Challenge Grant Challenge Grant. Browse more projects

Is habitat quality a key factor in determining whether koalas develop chlamydial disease?

By Amy Robbins
Backed by Suzanne Robbins, Sylvie Spark, BK Brad, Kathryn Mulheran, Renee Ford, Antony Smith, Fiona Ford, Wendy Benfer, Christina Sellick, Pat Forrestal, and 20 other backers
University of the Sunshine Coast
Australia
BiologyEcology
DOI: 10.18258/11690
Grant: Wildlife Disease Association Challenge GrantGrant: Wildlife Disease Association Challenge Grant
$5,037
Raised of $4,890 Goal
103%
Funded on 9/20/18
Successfully Funded
  • $5,037
    pledged
  • 103%
    funded
  • Funded
    on 9/20/18

DNA extraction and real-time polymerase chain reaction (qPCR)

DNA will be extracted from swabs samples according to the manufacturer's instructions in the QIAamp DNA mini kit. The extracted samples will be analysed using qPCR methods modified from Jelocnik et al. 2017.  The qPCR assays will be carried out in a total volume of 10μL, consisting of 5μL iTaqTM Universal SYBR® Green Supermix (Bio-Rad, Gladesville, New South Wales), 1.5μL DNA/RNA-free water, 0.5μL of each 10μM forward and reverse primer, and 2.5μL DNA template.  Samples will be run in duplicate, and positive and negative controls (DNA/RNA free water and miliqH2O) will be included in all qPCR assays.  

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