About This Project
Lung cancer, the largest cause of cancer mortality, accounts for 19% of all cancer deaths
Our previous experiment demonstrated the ability of carcinogen solvents to remove up to 65% of a carcinogen, b(a)p, from cells, as shown on the intro video
This experiment will test the hypothesis that carcinogen solvents, which reduce cellular carcinogen concentrations, will reduce genotoxic effects as measured by genomic analysis of treated vs untreated 3D pulmonary cell cultures
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What is the context of this research?
Lung cancer remains the largest cause of cancer death globally for several decades, accounting for nearly 20% of all cancer deaths (IARC factsheets). The vast majority of lung cancer patients, 85%-90%, are individuals exposed to tobacco smoke (IARC MONOGRAPHS). The remaining cases are correlated to occupational exposure to other carcinogens through different mechanisms.
What is the significance of this project?
Smokers develop lung cancer primarily because of an accumulation of chemical carcinogens. Because we know chemical carcinogens are the primary driver for lung cancer, the effective removal of chemical carcinogens may be a solution for reducing lung cancer rates. Chemical carcinogens become DNA adducts that lead to mutations in DNA. Our hypothesis is that reducing the concentration of chemical carcinogens in the lungs in a patient will reduce the incidence of lung cancer in that patient. To test this, we hypothesize that carcinogen exposed lung cells treated with carcinogen solvent liquids will show a lower genotoxic response compared to untreated cells.
What are the goals of the project?
To establish whether the removal of carcinogens from cells, using carcinogen solvents, reduces genotoxic effects of chemical carcinogens.
A pure model carcinogen and an environmentally relevant mixture of carcinogens/cocarcinogens and procarcinogens will be used to insult 3D lung tissue models.
Carcinogen solvents will subsequently be used to treat the insulted tissue models, and 24 hours later the tissue model cells will be collected for genomics analysis.
RNA sequencing will be used to establish all gene expression changes between the treated and un-treated experimental series.
The tissue models are 3D pulmonary tissue models which are the most relevant for chemical carcinogenesis as it relates to lung cancer.
Bench fees are for the preparation and treatment of the tissue models.
Genomics analysis will be done with a contract genomics centre who will be able to provide a full analysis of the effects of the treatment versus untreated controls.
This experiment will look at acute genotoxic effects, further experiments looking at chronic repeat genotoxic effects are also planned should this experiment validate the hypothesis that removing carcinogens has immediate anti-carcinogenic effects as measured by the proxy of genomic effects.
The project needs to be fully funded before the laboratory time can be booked adn scheduled.
Six to eight weeks for the 3d tissue culture work.
Six to eight weeks for genomics analysis.
Feb 04, 2018
Feb 18, 2018
Finalise protocol, choice of carcinogen mixtures
Mar 04, 2018
Complete 1st run of cell culture and carcinogen solvent treatments across all control and treatment series
Mar 18, 2018
Complete 2nd run of cell culture and carcinogen solvent treatments across all control and treatment series
Apr 01, 2018
Complete 3rd run of cell culture and carcinogen solvent treatments across all control and treatment series
Meet the Team
No results found for "biotech flaneur", so that's original at least.
A man who saunters around observing society.
French, from flâner ‘saunter, lounge’.
Mixed race, born in New York City, USA.
Child of the UN, literally, parents worked for the UNHCR and WHO, United Nations High Commissioner for Refugees and World Health Organisation.
Enjoyed playing sports, including rugby, rowing, cricket, basketball, and skiing.
Grew up near Geneva, Switzerland, and attended school in the United Kingdom.
Learnt to drive in Baghdad, Iraq, and visited Babylon, the ancient ruins not the TV set.
Studied biochemistry at Imperial College and London Metropolitan, developed interest in lung cancer research as I am a smoker.
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