Using the Ames test to test the effects of low frequency radiation on living systems

Princeton High School
Princeton, New Jersey
BiologyPhysics
DOI: 10.18258/14359
$1,586
Raised of $1,580 Goal
100%
Funded on 1/30/20
Successfully Funded
  • $1,586
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  • 100%
    funded
  • Funded
    on 1/30/20

About This Project

Low frequency radiation is a very common form of radiation in people's lives, two examples being cell phones and car radar emitters, used as safety features. Some studies have raised concerns over cell phones, but the available literature remains inconclusive. I am testing E.coli using the Ames test, a test for mutagenic substances, and fruit flies, using both cell phones and radar guns. I hypothesize there will be no change in the rate of mutations among cell phones and radar guns.

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What is the context of this research?

Some studies have raised safety concerns over low frequency radiation causing items, particularly cell phones. However, the literature seems to prove inconclusive, due to several factors, including the lack of human testing and the reliance of animal trials. Using the Ames test on E.coli and on fruit flies (Drosophila melanogaster), which both have shorter lifespans, can help serve as a more definitive model for testing whether low frequency radiation items do cause adverse effects. In this experiment, I will be testing using cell phones, and a radar gun to simulate car radar emitters. The Ames test is something which is new to the field, and fruit flies have only been used a limited number of times in these studies.

What is the significance of this project?

Cell phones and cars are some of the most common items in our lives. Seeing as there is a possibility that these items have adverse effects on human beings, the public should be informed as quickly as possible, as teenagers in particular spend hours a day on their phones, and many people own and drive at least one car, and if they do not are exposed to low frequency radiation when they are passed by a car. If my data provides support that there are no adverse effects, then there should be no need for safety precautions for using your cell phone, and the opposite is also true, if there are adverse effects, then there should be safety precautions put in place.

What are the goals of the project?

The overall aim of this project is to determine the effects of low-frequency radiation on living organisms. I hope to determine whether or not the cell phone and/or radar gun does cause mutation. The first portion of this study uses a version of the Ames test only requires biosafety level 1 protocols (due to E. coli). The low-frequency items will be tested and compared to a control, a known mutagen (UV light). All of the experimental plates will be placed in a shielded box to eliminate human exposure. A second aim is to determine whether distance from the radiation source changes the effect of low frequency radiation on organisms. If my results demonstrate that cell phones lead to increased mutation rates, limiting exposure to cell phones would be recommended to avoid impacts of radiation.

Budget

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To shorten the amount of time to collect data, the well plates would come prepped with the E.coli already in each slot of the well plate. The Incubator and Bio Hazard storage are to ensure that the E.coli in the well plates stay alive, and that they can be disposed of properly.

Since I am comparing responses between a prokaryotic organism and a multi cellular eukaryotic organism, I will need to order fruit flies and a E.coli culture kit.

For this experiment, I will be using my own phone and a radar gun, the latter will simulate the radar emitters in cars. As a positive control, I will be using UV light, which is a known mutagen.

Endorsed by

I have taught Ryan for several years, first as an elementary school science teacher, and later through accelerated chemistry and research methods and applications. Ryan is a very motivated investigator, and this experiment covers a topic that is very close to his heart. As a result, I am certain that he will successfully conduct this experiment to its completion, and will be doing all that he can to publicize his findings and grow as a researcher. Findings of this project will be relevant to everyone who uses a cell phone or related device.

Flag iconProject Timeline

Because E.coli and fruit flies have shorter life spans than many other organisms, trials will be able to progress quickly. The trials using E. Coli plates will have the low-frequency item placed a few inches over the well plates, and each trial will take place for around 3 weeks. The fruit fly trials will also have the low-frequency item placed a few inches over the culture kits, and will take place around 6 weeks, for at least two generations of fruit flies to be tested.

Dec 16, 2019

Project Launched

Jan 15, 2020

Obtain all materials and set up for all testing

Jan 22, 2020

Start Conducting Trial #1  for Cell Phones

Jan 22, 2020

Start Conducting Trial #1 for Radar

Feb 18, 2020

Start Conducting Trial #3

Meet the Team

Ryan Grzymala
Ryan Grzymala

Ryan Grzymala

Hello, my name is Ryan Grzymala, a high school junior at Princeton High School, and a part of Princeton High School's Research Class. Outside of doing my research I enjoy running, playing games, and playing the trumpet. This is my first research project, and I thank you for helping out on my project.

Additional Information

Endorsed by Dr. Celeste Nelson, Professor of Chemical and Biological Engineering, Director, Program in Engineering Biology, Princeton University

Endorsed by Dr. M. David Egger, Emeritus Faculty, Robert Wood Johnson Medical School Department of Neuroscience and Cell Biology.


Project Backers

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  • $58.74Average Donation
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