The Inverted Sequence (IR) of some plants is one of well-known kind of special sequences. But, until now, the exact mechanism of how the IR sequence is made hasn't discovered yet. Most plants have an IR regions, but legumes do not have an IR regions, and among them, deciduous legumes have an IR zone. Thus, the purpose of our study is to elucidate the structure and mechanism of the IR region. Additionally, our team is going to figure out the relationship of it and plants' deciduousness.