Methods
Summary
1. DNA synthesis of the NFAR-1B gene which is inserted into a pET15 expression plasmid with a His 6 tag on the N-Terminus followed by a thrombin cleavage site.
2. Transform into competent cells
3. Plate out on Amp-agar to grow colonies
4. Induce to express the peptide.
5. Purify by chromatography
6. Run SDS Page electrophoresis
Protocols
Browse the protocols that are part of the experimental methods.