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Can we express a synthetic gene of interest in bacteria for study as an antiviral? Schenderlein, Edwin, Celena Carrillo, Sean Greenwood, and Claudia Sanchez.. , 5 May 2016. Experiment
1. DNA synthesis of the NFAR-1B gene which is inserted into a pET15 expression plasmid with a His 6 tag on the N-Terminus followed by a thrombin cleavage site.
2. Transform into competent cells
3. Plate out on Amp-agar to grow colonies
4. Induce to express the peptide.
5. Purify by chromatography
6. Run SDS Page electrophoresis
Browse the protocols that are part of the experimental methods.