About This ProjectI'd like to make an IP-free expression vector for recombinant proteins to enable protein expression in 4 expression hosts with a single vector. This will make the expression of recombinant proteins for drug research easier, faster, and cheaper!
Results will be published with data in an open access, peer reviewed journal. In the end the new expression plasmid will be submitted to Addgene and DNASU, which will enable users all over the world.
Ask the ScientistsJoin The Discussion
What is the context of this research?
Typically scientists try to make proteins in a number of expression systems, each of which requires its own expression vector. It's pretty much trial and error to find out what expression system can produce a large amount of the target protein. In this project I propose to create and validate an expression vector that can express proteins in E.coli, insect cells, mammalian cells, and pichia pastoris. This vector should be IP free and able to be used by anyone, anywhere.
What is the significance of this project?
Recombinant protein production is the cornerstone of modern Biochemistry and Structural Biology. Being able to make these proteins in large amounts is often really useful for a variety of applications including structure-based drug design, immunization for vaccine and antibody discovery, and phage display. Similar vectors for three expression systems exist, but they are extremely expensive, which puts the time and cost savings provided by these vectors out of reach of the people that have the least money to spend.
What are the goals of the project?
I will use these funds to design and create the new vector, and then my clone a test set of 5 IP-free fluorescent proteins in single expression system vectors and then in the combined vector.
5 genes in E.coli
5 genes in Baculovirus infected insect cells
5 genes in mammalian
5 genes in pichia pastoris
5 genes in the multiple system expression vector
This means making a total of 25 expression constructs and testing them all in their respective systems, and then comparing all the results. These results will be published with data in an open access, peer reviewed journal. In the end the new expression plasmid will get uploaded to Addgene, which will allow anyone to download the sequence and receive a small aliquot of the plasmid so that they can use it in their own research.
My budget will be mainly used for the labor hours in designing the vector, cloning the 5 proteins, and expression testing all the resulting expression constructs.
Meet the Team
Team BioI am currently the founder of TimPharma LLC. I was previously a Project Lead at Emerald Bio, a protein biochemistry contract research organization, where I was a crystallographer for the Seattle Center for Structural Genomics of Infectious Diseases (SSGCID). You can view some of my past crystal structures on the Protein Data Bank website.
For a more detailed bio and examples of my work, please visit my Linkedin site.
I'm always interested in ways to make research projects easier and faster. I also really hate how IP laws have been abused to extract money from researchers and hinder the progress of science and medicine.
Postdoctoral Fellowship - Novartis/GNF
PhD in Molecular Biophysics - UT Southwestern Medical Center at Dallas
BS in Biochemistry and Neuroscience - Brandeis University
Brazilian Jiujitsu (I'm a blue belt)
I am currently a Project Lead at Emerald Bio, a protein biochemistry contract research organization. I'm also a crystallographer for the Seattle Center for Structural Genomics of Infectious Diseases (SSGCID). For the SSGCID, we try to get structures for difficult proteins involved in all kinds of diseases. You can view some of my past crystal structures on the Protein Data Bank website: http://www.pdb.org/pdb/author/Craig%2C+T.K.
- $795Total Donations
- $88.33Average Donation