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Detecção rápida do vírus Zika sem a necessidade de um laboratório no Brasil

Backed by Jose Francisco Gouvea Vieira, Sylvia Nabuco, Sylvia Braga, Duda Gouvêa Vieira, Lucia Rebello, Erik Sohn, Mark Hannaford, Murilo Dantas, Isha Datar, Gabriel Baldinucci, and 224 other backers
LIKA Laboratório de Imunopatologia Keizo Asami.
Brazil
BiologyMedicine
DOI: 10.18258/6724
Grant: Zika Virus
$29,345
Raised of $6,000 Goal
489%
Funded on 5/04/16
Successfully Funded
  • $29,345
    pledged
  • 489%
    funded
  • Funded
    on 5/04/16

Project Results

Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplifcation

Yohei Kurosaki, Danyelly Bruneska Gondim Martins, Mayuko Kimura, Andriu dos Santos Catena, Maria Amélia Carlos Souto Maior Borba, Sandra da Silva Mattos, Haruka Abe, Rokusuke Yoshikawa, José Luiz de Lima Filho, and Jiro Yasuda

About This Project

Vírus Zika (ZIKV) é uma pandemia em rápida expansão, uma emergência de saúde global. ZIKA tem sido associada commalformações do sistema nervoso central em recem-nascidos, juntamente com complicações neurológicas e imunes em adultos. Há uma escassez de laboratórios que oferecem testes para o ZIKV no Brasil e no mundo. Nós estamos desenvolvendo um novo teste de diagnóstico que identifica a presença de ZIKV e outros vírus. O objetivo é um teste fácil, rápido, econômico, local e confiável, sem a necessidade de um laboratório ou pessoal altamente treinado.

Ask the Scientists

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What is the context of this research?

Nós já começamos a desenvolver rapidamente e otimizar um teste específico e sensível para o ZIKA baseado na amplificação isotérmica de RNA genômico (Wang et al.) A partir de amostras minimamente tratadas (aquecida), sem a necessidade deum laboratório, equipamentos complexos ou pessoa altamente treinado. O teste será capaz de distinguir entre vírus estreitamenterelacionados, em menos de 60 minutos da extração da amostra, e os resultados facilmente visto. Vamos primeiro validar e otimizar o nosso conjunto de primers, usando genomas do ZIKA purificados, verificando também outros víruscirculantes, tanto o DNA humano quanto o do mosquito. Vamos então testar em vírus ZIKA cultivados em laboratório. Finalmentevamos testar em amostras humanas e de mosquitos, que temos acesso no laboratório LIKA no Brasil. Uma vez validado mais de 300 amostras vamos liberar o kit em um site aberto.

What is the significance of this project?

A situação atual no Brasil e na América Latina necessita urgentemente de um teste de diagnóstico, robusto, fácil de usar, e acessível para a detecção do vírus Zika. A estimativa atual quanto ao número de infecções é de mais de 1,5 milhões e subindo rapidamente. Existe uma correlação entre a infecção pelo vírus Zika, microcefalia (dimensão anormal da cabeça associada com o desenvolvimento cerebral incompleto) e síndrome de Guillain-Barre uma fraqueza muscular de início rápido, onde o sistema imune danifica o sistema nervoso periférico. No entanto o estado do diagnóstico atual é subdesenvolvido visto que o padrão ouro que é um teste de RT-PCRque não está disponível na maioria das clínicas, devido ao custo e a falta de equipamentos e pessoal treinado. A necessidade urgente de novos diagnósticos que são escaláveis, preciso e acessível foi descrito na OMS SRF (Fev 2016).

What are the goals of the project?

We have already started to rapidly develop, optimize and verify a specific and sensitive ZIKV test based on isothermal genomic RNA amplification (Wang et al.) from minimally treated samples (heated), without the need for a lab, complex equipment, or highly trained personnel. It will be able to distinguish between closely related viruses, in less than 60 minutes from sample extraction, and results easily seen.

We will first validate and optimize our primer set, using purified Zika genomes, also checking other co-circulating viruses, human and mosquito DNA. We will then test on ZIKV grown in the lab. Finally we will test on real samples of humans and mosquitoes, which we have access to at the LIKA lab in Brazil.

Once we validate >300 samples we will release the kit on an open website

Budget

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Já fizemos o trabalho in silico (computacional), analisando mais de 40 variantes do vírus ZIKA, e projetamos vários conjuntos de primers isotérmicos para diferentes regiões únicas do genoma ZIKA, levando em consideração as mutações recentes, relacionados com outros vírus e o genoma humano. Estamos prontos para comprar testá-los, só precisa de sua ajuda! A fim de otimizar o nosso ensaio e verificar que pode funcionar mesmo em ambientes de baixa renda, precisamos usar vários conjuntos de primers em muitos experimentos paralelos e em diferentes condições, concentrações de vírus, enzimas, pHs, temperaturas, tempo, utilizando diferentes técnicas de visualização e amostras. Custo de reagentes e disponibilidade são um passo limitante para nós, este financiamento vai permitir-nos trabalharmos mais rápido. Nós temos uma lista de materiais necessários, a fim de criar e validar o diagnóstico: cadeias curtas específicas de DNA (primers), micro-tubos de plástico, elemento de aquecimento, luvas, pipetas de plástico, enzimas, corantes e tampões.

Endorsed by

This project proposes to develop and validate a rapid, accurate field test for Zika infection, the first of its kind. The researchers running this campaign are well positioned to make a major impact on the clinical diagnosis of Zika virus infection, especially because the areas that have been the hardest hit are the least equipped to diagnose infection. The field test this group would design could be a turning point for similar areas impacted by the Zika outbreak.
This project aims at an essential and challenging goal: fast and cost-effective method to diagnose Zika virus infections in the field, without using laboratory equipment. As a synthetic biologist, I find this an elegant, simple and realistic solution a complicated problem. I highly recommend it. This can save many lives.
This project is addressing a topic critical for Brazil and other countries health system. Actions like that, trying to ease and speedup the disease detection, are crucial at this moment. The people involved are serious and combine the skills necessary to make a successful project.
This is a truly GREAT effort by a fantastic TEAM, aiming at an important, extremely RELEVANT GLOBAL public health PROBLEM of dire consequences for us all, specially the young ones and their families. It is a great honour to be both an endorser and supporter of this project. Silvio M., @srlm
This project has a powerful aim: to improve the point-of-care based in new approaches for diagnostics. As an expert in digital disease detection, for us it's very important to have platforms like that, helping to fight against epidemics, like Zika virus. Epitrack is completely favorable and we are glad to see this initiative as a scientific innovation applied to public health.
As a member of LIKA's research team , I find of great importance the development of this project . The Zika virus diagnostic will help in many ways to understand and prevent neurological and vascular complications. The relevance of the project and the skills of the researchers involved are fully endorsed by me.
I think this is an exciting and socially very needed project.
Knowing the researchers in person makes me very excited about this project, as I truly believe that this project has the ability to significantly aid in the combat against Zika in a timely fashion. I wish you all the best.
The ongoing outbreak of the Zika virus in Brazil and its alarming association with infant abnormalities, calls for better diagnostics and treatments for Zika. This project is of importance and timely, and the team of proffesionals lrading it are capable to meet the goal set by them. I gladly endorse them and wish them best of success.
This project is pretty amazing in showing how a group of people can come together in a short period of time to help deal with a serious issue.
I am happy to endorse this project as I believe it will be of great importance to shed light into this major public health problem.
The importance of this project needs no introduction. Right now, Zika is arguably the largest viral threat on the planet, and we know almost nothing about it. This team is proposing to develop a cheap, fast and high throughput method to identify Zika virus from patient samples – a critically important step for our understanding and eventual control of Zika. I have known Gilad for a number of years. His drive and motivation for innovative science and entrepreneurship is inspiring. I give this project my highest support.
I fully endorse and support this project. The team behind this project is doing an amazing job acting in loco on the ZIKA pandemic epicenter. Besides that, the concept of create a #lowcost #opensource diagnosis kit to be used by people in poor and rural areas is really breakthrough. As an investor in Synthetic Biology and Singularity University alumni (EP & GSP), projects like this increase my faith in humanity, and the power we have when we act together using exponential technologies to solve the greatest humanity problems.
This project addresses a real and urgent need in Zika-infected regions and beyond. Underlying the project is a potentially winning blend between state-of-the -art technology and it's wide open-source application that ensures its availability where it matters most.

Meet the Team

Gilad Gomé
Gilad Gomé
Principal Investigator

Affiliates

Singularity University, Tel Aviv University
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Danyelly Bruneska Gondim Martins
Danyelly Bruneska Gondim Martins
Scientist and Assistant Professor

Affiliates

Federal University of Pernambuco - UFPE
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Maria Amélia Borba
Maria Amélia Borba
Pharmacogenomics expert

Affiliates

Laboratory of Immunopathology Keizo Asami - Federal University of Pernambuco
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Henrique Castelletti
Henrique Castelletti
Genomics Scientist

Affiliates

Agronomic Institute of Pernambuco (IPA)
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Guy Aidelberg
Guy Aidelberg
Head of Science

Affiliates

Weizmann Institute of Science
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Alexander Kumar
Alexander Kumar
Clinical lead

Affiliates

University of Leicester; NIHR
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Jones Albuquerque
Jones Albuquerque
Computational Epidemiology Scientist

Affiliates

UFRPE - Statistical and Informatics Department / Epitrack
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Team Bio

The UK-IL-BR team got together in a short time to solve a big problem. Supported by a major research center in Brazil and advised by Universities, Hospitals and Impact organizations around the world.



Gilad Gomé

Biotech entrepreneur focused on scalable solutions for humanity's greatest challenges.

I am passionate about creating enabling platforms for promoting novel solutions that solve problems where technology allows new opportunities for the creation of for-benefit enterprises.

Danyelly Bruneska Gondim Martins

Ever since I can remember, I am fascinated by science! The curiosity moves me towards different themes and experiences to improve the knowledge and share the good ideas with cool people. In this way, we can make the science get closer to the society and the most needed communities and help them to improve their quality of life!

Maria Amélia Borba

PHD Student at LIKA. Has some experience in molecular diagnosis and pharmacogenomics. In love with science 24 hours a day!

Henrique Castelletti

A science/computer geek focused to find patterns in genome.
I am a dreamer about data integration as a platform to share knowledge and forwarding the development of life quality.


Guy Aidelberg

Broad range multidisciplinary scientist from Weizmann Institute with expertise in: physics, biology, data science. Author of research papers in fields of microfluidics, systems biology and microbiology.

Alexander Kumar

Alex is a British medical doctor currently working as a NIHR Academic Clinical Fellow in Infectious Diseases based at University of Leicester in United Kingdom. He has lived, worked and travelled through 80+ countries and holds experience working in an Ebola Treatment Centre in Sierra Leone and recently completed DTM&H at London School of Hygiene and Tropical Medicine.

Jones Albuquerque

holds a PhD in Computer Science. He is currently Associate Professor at the Federal Rural University of Pernambuco, Director of Knowledge of Epitrack, Chief Scientist of Innovation Senai Institute in Information and Communication Technologies. He has experience in Mathematics-Computer Software Engineering and modeling applied to Public Health, specifically in computational epidemiology and computational modeling.

Additional Information

Após a validação inicial, calibração e otimização, vamos testar o ensaio com nosso kit sem utilização de qualquer equipamento de laboratório. Isto irá assegurar que o ensaio pode ser feito em qualquer parte do mundo. Uma vez que valido mais de 300 amostras vamos liberar as especificações do kit para a OMS, OPAS, UNICEF e em um site de acesso aberto. O vírus Zika é um exemplo para muitos outros vírus que afetam o mundo e nosso ensaio pode ser usado em outros vírus emergentescom um processo de desenvolvimento rápido. Estamos Localizado no LIKA, Recife, Brasil e trabalhando no epicentro do vírus ZIKA espalhados em Pernambuco e Paraíba. Nossa equipe conta com professores e pesquisadores de LIKA, médicos e especialistas em doenças infecciosas do Reino Unido e cientistas de Israel, que se uniram para inovar 'aqui e agora' e fornecer soluções o mais rápido possível e que vai ajudar a resolver o problema. Seu apoio vai nos ajudar a ajudar os outros.

Project Backers

  • 273Backers
  • 489%Funded
  • $29,345Total Donations
  • $70.86Average Donation
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