Structural modeling of a key enzyme to combat late blight

$237
Pledged
20%
Funded
$1,200
Goal
7
Days Left
  • $237
    pledged
  • 20%
    funded
  • 7
    days left

About This Project

Phytophthora infestans is the causal agent of late blight disease, which affects potato and tomato crops, causing significant economic losses in the production of these crops worldwide. Glucokinase 1 from P. infestans (PiGlcK-1) is a key enzyme in the metabolism of this phytopathogen. In this project we propose to make a structural characterization of PiGlck-1, which could contribute to the design of inhibitors for this enzyme that help to combat this disease.

Ask the Scientists

Join The Discussion

What is the context of this research?

Previously, we have found that PiGlck-1, one of the 7 glucokinases present in P. infestans, can phosphorylate both glucose and fructose, using ATP, ADP or PPi as phosphoryl group donors, attributing to this enzyme an important role during the establishment of infection.

For this reason, we believe that a sturctural characterization of this enzyme will provide information on the potential sites on which to design inhibitors of the late blight pathogen.

Late blight is a oomycota disease that is feared by farmers and industries. It rapidly and completely destroys potato and tomato crops during the growing season, and even during storage.


What is the significance of this project?

Late blight is one of the most devastating diseases in the world, attacking potato tubers and tomato fruit. Many of the strategies to control this disease continue to be based on the indiscriminate use of fungicides, but these generate environmental pollution, health risks and increase in production costs.

A potential target of attack to combat this disease could be focused on key enzymes of the metabolism of P. infestans. PiGlck-1 it has a significant degree of expression in the forms responsible for establishing infection in plants. Therefore, a more detailed study on this enzyme would contribute to the design of strategies to prevent greater economic losses in the production of these crop, prevent the use of fertilizers harmful to the environment and people's health.

What are the goals of the project?

The objectives set by our team consist of modeling and refining the three-dimensional structure of PiGlck-1 by homology, using different servers that allow obtaining the best tempering. Subsequently, we wish to know through molecular docking the binding sites for glucose, fructose, ATP, ADP and PPi ligands. Likewise, we propose to publish the results obtained through this project, in an international open access journal, in order to contribute to the advancement towards knowledge within the metabolism of P. infestans for the design of the most effective strategy against this threat.

Budget

Please wait...

This work is 80% completed, structural models of the protein are currently being carried out, lacking the determination of the ligand binding sites. The funds raised will be used to finalize the computational analysis of the modeling, as well as the writing and submission for the publication of the results in an Open Access journal.

Endorsed by

This project is very interesting, relevant and achievable. The researchers involved know what they need and are fully trained to do it. The work also represents a necessary contribution with repercussions in phytopathology and food safety, of great relevance for Venezuela and the world at this time.

Flag iconProject Timeline

This work began at the end of last year, having completed the structural studies in January 2021. We hope to carry out an analysis of the results obtained by May of this year and to draft the manuscript for submission to an open access journal in June of 2021.

Oct 22, 2020

Start of the project

Jan 15, 2021

Structural studies

Apr 14, 2021

Project Launched

May 01, 2021

Analysis of results

Jun 01, 2021

Drafting the manuscript and sending it to the journal

Meet the Team

Liara Villalobos-Piña
Liara Villalobos-Piña
Student

Affiliates

Center for Scientific Calculus, University of Los Andes (CeCalCULA)
View Profile
Ascanio Rojas A.
Ascanio Rojas A.
Associate Professor

Affiliates

Center for Scientific Calculus of the University of Los Andes (CeCalCULA)
View Profile
Hector Acosta Oviedo
Hector Acosta Oviedo
Doctor

Affiliates

Universidad de Los Andes
View Profile

Team Bio

The Scientific Calculation Center of the University of Los Andes is a multidisciplinary research group that develops its line in the study of human and plant pathogens, mainly Trypanosomatides, Apicomplexa and Oomycetes. This
center develops lines of research such as biochemistry, population genetics and computational biology, all of them applied to these pathogens

Liara Villalobos-Piña

I am a biologist, graduated from the Faculty of Sciences of the University of Los Andes, Venezuela. My line of research since 2017, it has been developed especially in the area of Biochemistry, specifically in the study of the metabolism of Phytophtora infestans, characterization and enzymatic kinetics as well as the overexpression and purification of recombinant glucokinases of this model.

I am currently researching, independently, from the area of computational biology using P. infestans and other pathogens as a study model. My main interest is to study its pathogenicity mechanisms, as well as to structurally characterize some of the key enzymes involved in the host-pathogen interaction that are potential targets of attack in the design of future strategies aimed at the control of diseases of economic interest.

Ascanio Rojas A.

Director of Center for Scientific Calculus, University of Los Andes (CeCalCULA), Merida - Venezuela.

We do research in computational biology applied to the epidemiology, evolution and molecular population genetics of human parasites, focusing mainly on Trypanosoma cruzy and Plasmodium vivax, which cause Chagas disease and malaria, respectively. In addition, we train young researchers in these areas by teaching biannual courses in bioinformatics, evolution and biochemistry.

Interests: Computational Biology, Population Genetics, Molecular Phylogenetics and Evolution, Genomics and Bioinformatics courses

Hector Acosta Oviedo

Full-time professor in the Department of Biology of the Faculty of Sciences (Universidad de Los Andes, Venezuela) with a Master's degree in Fundamental Medical Sciences and a PhD in Cell Biology (Universidad de Los Andes, Venezuela). Researcher since 1997. I have developed my line in the area of Biochemistry, carrying out intermediate metabolism, enzyme purification and enzyme kinetic studies in Trypanosomatidae, specifically in Trypanosoma cruzi, Trypanosoma evansi, Trypanosoma rangeli and Leishmania mexicana (ULA) and Trypanosoma brucei (Victor Segalen University, France) as well as Phythoptora infestans, Plasmodium falciparum and members of the genus Pseudomonas.


Project Backers

  • 4Backers
  • 20%Funded
  • $237Total Donations
  • $59.25Average Donation
Please wait...

See Your Scientific Impact

You can help a unique discovery by joining 4 other backers.
Fund This Project
Project journal