ZILLION: A novel zebrafish model for studying inflammation and infection in Inflammatory Bowel Disease

Single injury protocol is adapted and modified from Oehlers et. al. 2013. In short; batches of 60 larvae were segregated into Petri dishes in 50 mL egg water. Phenylthiourea (75 μM; Acros Organics) was added 24 hpf to prevent pigment cell formation. To induce intestinal injury, 3 day-post fertilization (dpf) larvae were placed in freshly prepared 0.1 or 0.25% (w/v) colitis grade DSS (36,000 - 50,000 MW, MP Biomedical) for three days. In repeated injury protocol, larvae were with 0.05, 0.1 or 0.25% DSS at 5, 8, 11 dpf for 24 hours.