Bowen Jiang

Bowen Jiang

4000 Edison Avenue, Sacramento, CA 95821

Mira Loma High School

Independent High School Researcher


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Published on Dec 12, 2017

Project update, future research ideas + more

Hello everyone, So it's been a pretty productive several weeks for me in the lab. Thanks to a one-week break with no school, I was able to finish my final rounds of PCR, set up my sample and primer...

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Published on Nov 18, 2017

Algae at high school??

Yes, I am indeed this much of a nerd. But that's not really the point here. So, high school. Instant thoughts: "...musical", "ugh calculus", "pre-adult freedom" (enjoy it while you can). Yet have y...

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Published on Nov 16, 2017

A note on electrophoresis in my lab

Hello everyone, So as I wrap up the final PCRs for my current algal strains in preparation for my second round of DNA sequencing, I thought I'd go over how I perform electrophoresis, an essential s...

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Published on Nov 04, 2017

JIAC26+ : Introducing some new strains to this study

Hello everyone, So I mentioned in some of my previous lab notes that I'm still working on PCR, which are actually pretty much all done by this time. Many of these reactions, most notably those for ...

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Published on Oct 30, 2017

Project Update - Current Progress, Continuing Work, Future Plans

Hello everyone, So seeing as I've been updating my project mainly on smaller details or sub-projects, I figured I'd do a update on this study as a whole, recapping what's already been done, what's ...

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Published on Oct 25, 2017

Sequence Data - short follow-up with some more discussion

Just a quick update: all of the 12 sequences mentioned in yesterday's lab note are up on GenBank and available to view now. I checked several times throughout the day for the two non-rRNA sequences...

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Published on Oct 24, 2017

Sequence data finally online - includes new genes for TWO genera!

Hello everyone, So, this current update on the DNA sequences that I sent to GenBank for accession comes a couple of weeks later than I initially anticipated. Of the 12 sequences that were included ...

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Published on Oct 05, 2017

Sub-project Sequences to GenBank

Hello everyone, For the past week or so, it's been difficult for me to get very much work done on my project, especially experiments in the lab. I've been slowly working on some of the computer seq...

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Published on Sep 23, 2017

The Algae Doctor - Brief Notes on Antibiotic Treatment for Axenic Algal Strains

Hello everyone, So the "first phase" of my project is currently wrapping up...I've gotten lots of sequence data for the 33 strains of collected and cultured algae I've been working with for most of...

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Published on Sep 15, 2017

Phylogenetic Trees, v2.0 - Slightly More Polished Data

A little less than two weeks ago, I published a lab note detailing the first preliminary tree data I've managed to compile and analyze, including around two-and-a-half dozen partial sequences of th...

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Published on Sep 02, 2017

1st Real Tree - Super-Preliminary rbcL Data!

Hello everyone, So, as promised in my last lab note, I have my first phylogenetic trees that I've been able to create with some of my rough sequences. Just in case I haven't stressed this enough - ...

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Published on Aug 30, 2017

Quick Update on Strain Identities

Hello everyone, I've now been sitting on my sequence data for a good couple of weeks, slowly working on editing them and stitching together partial sequences (as some of my barcode regions are too ...

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Working on this project as a whole has definitely given me a greater appreciation for the difficulties of phylogenetic research. Of course, most of my lab notes at this time have been written with my personal assumption that the data presented are preliminary and not as accurate or precise as they will be upon further processing, but I do appreciate that you have noticed that my tone does not always reflect this, something which I will make sure to correct in future lab notes. I am currently trying to establish connections with a local college where I can get more direct and technical support on my methods and data processing, and I also have contact with a few algal phylogeneticists whom I plan to speak to more when I dive deeper into the analysis of my data, so especially as I move beyond the routine algal isolation and PCR I run at home I will definitely search for more assistance on the computerized portions of my project. I was wondering, however, if you would be able to assist me a little as well by perhaps helping me glance over some of my lab notes or speak personally about the goals and aims of my project. I would love to receive more of your input to figure out how I can continue to improve this project as well as word my lab notes so that it is more clearly presented to others. Thanks again for your feedback, and I hope to hear from you more as I continue to work on my research! Best regards, Bowen
Aug 19, 2017
The Ideal Molecular Barcode for Identifying Freshwater Green Algae (Chlorophyceae)
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Hello Dr. Hall, Thank you very much for your quick comment and continued interest in my project! Throughout most of my lab notes, I have been struggling with striking a balance between maintaining a readable and interesting vocabulary and note length and going into more detailed or complicated explanations of my methods, so I apologize as I do not think I did a very good job of explaining my use of BLAST and sequence editing software in this lab note. By calling BLAST subjective, I was referring to my making the subjective choices of which sequences to consider editing based off of the algorithmic and quantitative process by which the alignments are generated and scored, not the process of BLAST itself. The “unnecessary sequences” you refer to in your comments are simply the long tails of low-quality uncalled bases in each chromatogram that I think are artefacts of the capillary electrophoresis runs (e.g. for a 700-bp product, I would trim off the additional 700 base “tail” of mostly “N” bases at the end). However, upon rereading my lab note, I can see how my wording would have led to such ambiguity and misinterpretation, so I will work to get that edited as soon as possible.
Aug 19, 2017
The Ideal Molecular Barcode for Identifying Freshwater Green Algae (Chlorophyceae)
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Hello Dr. Hall, As I have been having trouble with commenting on my lab note, please see the message I have directly sent you. Thanks! Best regards, Bowen Jiang
Aug 19, 2017
The Ideal Molecular Barcode for Identifying Freshwater Green Algae (Chlorophyceae)
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Hello Dr. Hall, As I have been having trouble with writing direct replies to comments, please see the second comment on this lab note. Thanks! Best regards, Bowen Jiang
Aug 19, 2017
The Ideal Molecular Barcode for Identifying Freshwater Green Algae (Chlorophyceae)
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Hello, congratulations on full funding for your project! I am curious what species of cyanobacteria are most prevalent in the areas which you are studying. The mention of anatoxin-a makes me instinctively think Anabaena, but the pictures in our video seem to show benthic growth, so could it possibly be an Oscillatoria or Nostoc species also? Thank you for your research, and I am excited to see how this project progresses!
Apr 30, 2017
When Does the Eel River Turn Toxic? Patterns in Cyanotoxin Occurrence 2013-2016
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