Results of a Crowd-Funded Experiment to Express a Recombinant Protein (GFP) in the Chloroplast of the Green Alga, Chlamydomonas reinhardtii.
Kwang-Chul Kwon, Antonio Lamb, Simon Porphy, Sebastian Cocioba
About This Project
This page serves as an archive of our first proof-of-concept experiments to genetically modify microalgae before founding MicroSynbiotiX. We were partially successful in expressing proinsulin. We were successful in genetically modifying a strain of microalgae to express recombinant proteins (GFP), fish vaccines, and we even began fish vaccine trials with our first candidate product. Fish vaccines are our priority now, but we will revisit insulin and human therapeutics soon!
Ask the ScientistsJoin The Discussion
What is the context of this research?
I was inspired to pursue this project when I made contact with two other individuals, Simon Porphy, a Fermentation Scientist with a Master’s Degree in Bioprocess Engineering, and Jeffrey Boore, a UC Berkeley genomics professor and former panel adviser for the National Science Foundation. They informed me of the untapped potential of microalgae and their intent to harness it, and so we all decided to collaborate. Numerous studies have shown that vaccine production in algae is on the brink of becoming an established technology . I want to provide public exposure for this new development and demonstrate that with the right team, it will be ready for industry. I want to use microalgae to produce therapeutic proteins and bring down their cost. I seek your help to do so.
What is the significance of this project?
According to the World Health Organization and the American Diabetes Association, cholera and diabetes are treatable diseases that take the lives of approximately 376,000 people worldwide each year . Imagine the number of lives that could be saved by using plants to develop cheap edible vaccines, or using algae to grow more affordable synthetic insulin for diabetics in disadvantaged countries. The benefits to developing nations would be tremendous. I have assembled a highly qualified team of scientists to help me, and they have many years of research and industry experience with microalgae. With your help, we can use our talents to try and make a positive contribution to society. It is also a great opportunity to show how powerful crowd-sourced scientific research can be.
What are the goals of the project?
I want to use this pilot study to demonstrate that protein production in algae can be more economically viable than traditional methods using bacterial or mammalian cells. I have designed a new gene to produce synthetic insulin in algae which does not rely on photosynthesis. Additional details of the experimental design (for those familiar with molecular biology) will be available. Essentially, I will be inserting a gene encoding synthetic proinsulin into the chloroplasts of microalgae, where it should accumulate to high concentrations without killing the cell. A similar experiment showed promising results .
UPDATE: Since we were able to meet our funding goal thanks to the incredible generosity of friends and strangers, we can go beyond the bare minimum. We would like to use any additional funds to order more DNA primers, additional algae strains from the chlamy center, more glassware, and an additional shaker. We would also like to synthesize a few more variants of our synthetic proinsulin gene, and an antigenic viral epitope for a fish vaccine down the line if our algae insulin results are promising. It would also be nice to get a genomic DNA extraction kit so we can purify the DNA professionally and store it as a library, and maybe not skimp on having all the necessary chemicals to build our own media variation and buffers so we don’t have to buy expensive pre-made media each time. The budget of this pilot study is critical for successfully completing the experiment. It is needed to directly fund the demonstration that our ideas work.
Meet the Team
We are a group of passionate and educated scientists with several years of industry and academic experience. We value transparency and honesty, which we will maintain throughout the experimental process.
I am a scientist trained in plant and bacterial molecular genetics. I am competent in plant tissue culture, agrobacterium-mediated genetic engineering, and microalgae plastid engineering.
Simon Jegan Porphy, Jegathese
I am a Scientist with expertise in Fermentation research. I bring strong entrepreneurial drive and passion for biotechnology. All discovery happens by serendipity but for commercial success we need structure, planning and execution. I bring my engineering and science skills into the project to make it successful. Almost any idea can be demonstrated on a lab scale. However, good engineering skills are required to understand scale-up challenges and study the feasibility of a process. I hold more than 4 years of Industry work experience. Previously worked with DuPont and Life Technologies Also, I have first authored, peer-reviewed publication on studying the feasibility of microalgae biomass production for biofuels.
I will be helping maintain the experiments, analyzing data, and assisting with the transformation protocols.
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