Your funding will help me pay the hourly fee for the time needed on state-of-the-art equipment to collect the fungal cells out of frozen infected ant heads that have been manipulating the brain (cryosectioning and laser capture microscopy).
From these cells RNA will be extracted and amplified towards DNA libraries to be able to look at the expression of genes that are in play. Part of the funding will therefore be used to buy kits for this.
Subsequently, these libraries will be used for RNA sequencing. Two lanes of the sequencer will be used which will be sufficient to run all 5 biological replicates of each of the 3 sample types. A big part of the funding will thus go towards this.
Finally, I will analyze the data. My time is paid for by my Marie Curie Fellowship so your funding will solely be used to pay for equipment time, sample preparation and sequencing.
This will result in an immense amount of data that will answer the research question proposed here, but will also be greatly informative for future projects.